Structure and splice products of the human gene encoding sds22, a putative mitotic regulator of protein phosphatase-1
Structure and splice products of the human gene encoding sds22, a putative mitotic regulator of protein phosphatase-1
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DOI：
10.1046/j.1432-1327.1999.00344.x
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发表时间：
1999-05-01
期刊：
影响因子：
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通讯作者：
Bollen, M
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文献类型：
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作者：
Ceulemans, H;Van Eynde, A;Bollen, M
SDS 22是蛋白磷酸酶-L的调节亚基，其是酵母中完成有丝分裂所必需的。它主要由22个残基的11个串联富含亮氨酸的重复序列组成，预期这些重复序列可介导与醚多肽（包括蛋白磷酸酶-1）的相互作用。在本文中，我们报告的结构的人类基因编码sds 22，指定PPP 1 R7。该基因（33 kb）包含11个外显子，但这些外显子与编码富含亮氨酸重复序列的序列不一致。表达序列标签数据库分析、RT-PCR和北方印迹分析表明，通过外显子跳跃和选择性多聚腺苷酸化可以产生多达六种剪接变体。预期sds 22转录物编码四种不同的多肽。sds 22 alpha（1）对应于先前从人脑克隆的变体[Renouf等（1995）FEES Lett. 375，75-78]。Sds 22 β（1）在第九个重复序列内被截短，并具有短而不同的C末端。这两种变体也存在没有对应于外显子2的序列，并且它们被称为sds 22 alpha（2）和sds 22 beta（2）。PPP 1 R7的5 '侧翼区在转录起始位点附近含有两个NF-Y结合CCAAT盒，并含有转录因子c-Myb、Ik-2和NF-1的潜在结合位点，这些转录因子在小鼠基因中是保守的。
sds22 is a regulatory subunit of protein phosphatase-l that is required for the completion of mitosis in yeast. It consists largely of 11 tandem leucine-rich repeats of 22 residues that are expected to mediate interactions with Ether polypeptides, including protein phosphatase-1. In this paper, we report on the structure of the human gene encoding sds22, designated PPP1R7. This gene (33 kb) comprises 11 exons, but these do not coincide with the sequences encoding the leucine-rich repeats. Up to six splice variants can be generated by exon skipping and alternative polyadenylation, as revealed by expressed sequence tag database analysis, RT-PCR and Northern blot analysis. The sds22 transcripts are expected to encode four different polypeptides. sds22 alpha(1) corresponds to the variant cloned previously from human brain [Renouf et al. (1995) FEES Lett. 375, 75-78]. Sds22 beta(1) is truncated within the ninth repeat and has a short and different C-terminus. Both variants also exist without the sequence corresponding to exon 2, and these are termed sds22 alpha(2) and sds22 beta(2). The 5'-flanking region of PPP1R7 contains two NF-Y-binding CCAAT boxes near the transcription start site and potential binding sites for the transcription factors c-Myb, Ik-2 and NF-1, which are conserved in the mouse gene.