Cross-talk between diverse serine integrases.
Cross-talk between diverse serine integrases.
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不同丝氨酸整合酶之间的串扰。
DOI：
10.1016/j.jmb.2013.10.013
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发表时间：
2014
影响因子：
5.6
通讯作者：
Hatfull,GrahamF
中科院分区：
文献类型：
--
作者：
Singh,Shweta;Rockenbach,Kate;Dedrick,RebekahM;VanDemark,AndrewP;Hatfull,GrahamF
Phage-encoded serine integrases are large serine recombinases that mediate integrative and excisive site-specific recombination of temperate phage genomes. They are well suited for use in heterologous systems and for synthetic genetic circuits as theattPandattBattachment sites are small (< 50 bp), there are no host factor or DNA supercoiling requirements, and they are strongly directional, doing only excisive recombination in the presence of a recombination directionality factor. Combining different recombinases that function independently and without cross-talk to construct complex synthetic circuits is desirable, and several different serine integrases are available. However, we show here that these functions are not reliably predictable, and we describe a pair of serine integrases encoded by mycobacteriophages Bxz2 and Peaches with unusual and unpredictable specificities. The integrases share only 59% amino acid sequence identity and theattPsites have fewer than 50% shared bases, but they use the sameattBsite and there is non-reciprocal cross-talk between the two systems. The DNA binding specificities do not result from differences in specific DNA contacts but from the constraints imposed by the configuration of the component half-sites within each of the attachment site DNAs.
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