Serum contains chemoattractants for human retinal pigment epithelial cells.
Serum contains chemoattractants for human retinal pigment epithelial cells.
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DOI：
10.1001/archopht.1984.01040031488029
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发表时间：
1984-12
影响因子：
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通讯作者：
P. Campochiaro;J. Jerdan;B. Glaser
中科院分区：
文献类型：
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作者：
P. Campochiaro;J. Jerdan;B. Glaser
玻璃体腔中的血液或血清与增殖性玻璃体视网膜病变和严重创伤后的细胞膜形成有关。视网膜色素上皮细胞（RPE）是这些膜的重要组成部分。为了使 RPE 细胞有效地扩散到整个玻璃体腔并形成收缩膜，必须发生细胞迁移。我们发现胎牛血清和人血清以剂量依赖性方式增加人RPE细胞迁移；纤连蛋白 (FN) 导致 RPE 细胞迁移出现类似程度的剂量依赖性增加。该效应独立于对细胞附着的任何影响。 RPE 细胞迁移沿着从低浓度到高浓度的 FN 的梯度发生，因此代表趋化性。 FN 耗尽的血清失去了刺激 RPE 细胞迁移的大部分能力。这些结果表明，血清含有人类 RPE 细胞的化学引诱剂，并且 FN 在很大程度上发挥了这种活性。
Blood or serum in the vitreous cavity is associated with cellular membrane formation in proliferative vitreoretinopathy and following severe trauma. Retinal pigment epithelial cells (RPE) are important components of these membranes. For RPE cells to effectively spread throughout the vitreous cavity and form contractile membranes, cell migration must occur. We found that fetal bovine serum and human serum increase human RPE cell migration in a dose-dependent manner; fibronectin (FN) caused a dose-dependent increase in RPE cell migration of similar magnitude. This effect was independent of any effect on cell attachment. The RPE cell migration occurred along a gradient from low to high concentrations of FN and thus represents chemotaxis. Serum depleted of FN lost much of its ability to stimulate RPE cell migration. These results demonstrate that serum contains chemoattractants for human RPE cells and that FN accounts for much of this activity.