USE OF SITE-DIRECTED MUTAGENESIS OF ALLELE-SPECIFIC PCR PRIMERS TO IDENTIFY THE GSTM1-A, GSTM1-B, GSTM1-A,B AND GSTM1 NULL POLYMORPHISMS AT THE GLUTATHIONE-S-TRANSFERASE, GSTM1 LOCUS
USE OF SITE-DIRECTED MUTAGENESIS OF ALLELE-SPECIFIC PCR PRIMERS TO IDENTIFY THE GSTM1-A, GSTM1-B, GSTM1-A,B AND GSTM1 NULL POLYMORPHISMS AT THE GLUTATHIONE-S-TRANSFERASE, GSTM1 LOCUS
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DOI：
10.1042/bj2950313
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发表时间：
1993-10-01
影响因子：
4.1
通讯作者：
STRANGE, RC
中科院分区：
文献类型：
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作者：
FRYER, AA;ZHAO, L;STRANGE, RC
用一步聚合酶链式反应方法检测谷胱甘肽S转移酶GSTM1基因座的GSTM1零、GSTM1A、GSTM1B和GSTM1A、B多态。用内含子6和外显子7的引物扩增靶DNA，用定点突变在GSTMI*A扩增的DNA中引入限制酶切位点，从而使该等位基因与GSTM1*B区分开来。通过与已建立的区分GSTM1*0纯合子和其他GSTM1表型的聚合酶链式反应方法的比较，证实了该方法在鉴定GSTM1 A、GSTM 1 B、GSTM 1 A、B和GSTM 1缺失多态方面的准确性。
We describe the identification of the GSTM1 null, GSTM1 A, GSTM1 B and GSTM1 A,B polymorphisms at the glutathione S-transferase GSTM1 locus using a single-step PCR method. Target DNA was amplified using primers to intron 6 and exon 7 with site-directed mutagenesis being used to introduce a restriction site in DNA amplified from GSTMI *A, thereby allowing differentiation of this allele and GSTM1 *B. The accuracy of this approach in identifying the GSTM1 A, GSTM 1 B, GSTM 1 A,B and GSTM 1 null polymorphisms was confirmed by comparison with, firstly, an established PCR method that distinguishes GSTM1 *0 homozygotes from individuals with the other GSTM1 genotypes and, secondly, GSTM1 phenotypes determined using chromatofocusing.