ISOLATION OF A PROTEIN THAT IS ESSENTIAL FOR THE FIRST STEP OF NUCLEAR-PROTEIN IMPORT
ISOLATION OF A PROTEIN THAT IS ESSENTIAL FOR THE FIRST STEP OF NUCLEAR-PROTEIN IMPORT
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DOI：
10.1016/0092-8674(94)90067-1
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发表时间：
1994-12-02
期刊：
影响因子：
64.5
通讯作者：
HARTMANN, E
中科院分区：
文献类型：
--
作者：
GORLICH, D;PREHN, S;HARTMANN, E
我们已经从非洲爪蟾卵中纯化了一种细胞溶质蛋白，这种蛋白对于选择性蛋白质输入细胞核是必不可少的。纯化的蛋白质，命名为importin，促进信号依赖性结合的亲核蛋白的核膜。我们已经克隆、测序并表达了相应的cDNA。Importin与SRP 1 p（一种与酵母核孔复合物相关的蛋白质）显示出44%的序列同一性。通过结合从非洲爪蟾卵中纯化的或在E.大肠杆菌Ran/TC 4。提供了其他刺激因素的证据。
We have purified a cytosolic protein from Xenopus eggs that is essential for selective protein import into the cell nucleus. The purified protein, named importin, promotes signal-dependent binding of karyophilic proteins to the nuclear envelope. We have cloned, sequenced, and expressed a corresponding cDNA. Importin shows 44% sequence identity with SRP1p, a protein associated with the yeast nuclear pore complex. Complete, signal-dependent import into HeLa nuclei can be reconstituted by combining importin purified from Xenopus eggs or expressed in E. coli with Ran/TC4. Evidence for additional stimulatory factors is provided.