Betulin suppresses S. aureus-induced mammary gland inflammatory injury by regulating PPAR-γ in mice
Betulin suppresses S. aureus-induced mammary gland inflammatory injury by regulating PPAR-γ in mice
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DOI：
10.1016/j.intimp.2015.08.035
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发表时间：
2015-12-01
影响因子：
5.6
通讯作者：
Deng, Ganzhen
中科院分区：
文献类型：
--
作者：
Guo, Meng-yao;Li, Wen-yu;Deng, Ganzhen
乳房炎是一种人类和动物的产后疾病。金黄色葡萄球菌(S.aureus)可通过感染泌乳管引起乳房炎。目前还没有有效的治疗金黄色葡萄球菌引起的乳房炎的方法。白桦醇已被证实具有多种生物活性，包括抗炎特性。本研究旨在探讨白桦醇对金黄色葡萄球菌所致乳房炎的抗炎作用及其作用机制。在体内，桦木醇能改善金黄色葡萄球菌引起的组织病理学改变。酶联免疫吸附试验和定量聚合酶链式反应结果显示，白桦醇可抑制肿瘤坏死因子-α、白介素1β和白介素6的产生。Western blotting结果显示，桦木醇抑制了NF-kappaB的磷酸化，但促进了PPAR-γ的表达。用小鼠乳腺上皮细胞(MMECs)进行了进一步的体外研究。结果表明，白桦醇抑制了核因子-kappaB途径的活性，增加了PPAR-γ的表达和转录活性。本研究的所有结果表明，白桦醇通过激活PPAR-γ和抑制NF-kappa B的激活，对乳腺组织和细胞中的金黄色葡萄球菌感染具有保护作用。(C)2015 Elsevier B.V.保留所有权利。
Mastitis is a postpartum disease in both humans and animals. Staphylococcus aureus (S. aureus) can induce mastitis by infection of the lactiferous ducts. There is no efficacious treatment for S. aureus-induced mastitis. Betulin has been confirmed to have multiple biological activities, including anti-inflammatory properties. The present study was to determine the anti-inflammatory effect of betulin on S. aureus-induced mastitis and to confirm the mechanism of action involved. In vivo, betulin ameliorated the histopathological changes that were induced by S. aureus. ELISA and qPCR results showed that betulin inhibited TNF-alpha, IL-1 beta and IL-6 production. Western blotting results demonstrated that betulin inhibited NF-kappa B phosphorylation but promoted the expression of PPAR-gamma. Further investigations were performed in vitro with mouse Mammary Epithelial Cells (mMECs). The results indicated the betulin inhibited the activity of the NF-kappa B pathway and increased PPAR-gamma expression and transcriptional activity. All of the results in the present study demonstrated that betulin played a protective anti-inflammatory role against S. aureus infection in mammary gland tissues and cells by activating PPAR-gamma and inhibiting the activation of NF-kappa B. (C) 2015 Elsevier B.V. All rights reserved.