Negative staining and adenosine triphosphatase activity of annulate lamellae of newt oocytes.
Negative staining and adenosine triphosphatase activity of annulate lamellae of newt oocytes.
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DOI：
10.1083/jcb.42.2.519
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发表时间：
1969-08
影响因子：
7.8
通讯作者：
Franke, W W
中科院分区：
文献类型：
--
作者：
Scheer, U;Franke, W W
通过改进的 Callan-Tomlin 技术从单个蝾螈卵母细胞（Triturus alpestris）制备半分离的环形片层。用电子显微镜检查此类制品，并描述了环形薄片的负染色外观。可以检测到环形片层粘附在核膜上或与核膜分离。有时观察到它们与细长的管状结构相连，被解释为内质网的一部分。将阴性染色获得的结果与切片的结果结合起来。特别是，比较了同一细胞的环形片层和核膜的结构数据。有证据表明，在所研究的卵母细胞中，两种多孔池，即环形片层和核膜，显着区别在于环形片层表现出高得多的孔频率（通常约为相应核膜的两倍），并且还具有占据池表面的高达 32% 至 55% 的相对孔面积（相比之下，核膜中的孔频率为 13% 至 22%）。然而，两种多孔水池的孔径和孔隙复合体的所有其他超微结构细节是相同的。与各种动物和植物细胞的核孔复合体的环形一样，环形片层孔的环形也揭示了其亚基在负染色和切片材料中的八重对称性。此外，环形薄片被证明是 Mg-Na-K 刺激的 ATP 酶的活性位点。
Semi-isolated annulate lamellae were prepared from single newt oocytes (Triturus alpestris) by a modified Callan-Tomlin technique. Such preparations were examined with the electron microscope, and the negative staining appearance of the annulate lamellae is described. The annulate lamellae can be detected either adhering to the nuclear envelope or being detached from it. Sometimes they are observed to be connected with slender tubular-like structures interpreted as parts of the endoplasmic reticulum. The results obtained from negative staining are combined with those from sections. Especially, the structural data on the annulate lamellae and the nuclear envelope of the very same cell were compared. Evidence is presented that in the oocytes studied the two kinds of porous cisternae, namely annulate lamellae and nuclear envelope, are markedly distinguished in that the annulate lamellae exhibit a much higher pore frequency (generally about twice that found for the corresponding nuclear envelope) and have also a relative pore area occupying as much as 32% to 55% of the cisternal surface (compared with 13% to 22% in the nuclear envelopes). The pore diameter and all other ultrastructural details of the pore complexes, however, are equivalent in both kinds of porous cisternae. Like the annuli of the nuclear pore complexes of various animal and plant cells, the annuli of the annulate lamellae pores reveal also an eightfold symmetry of their subunits in negatively stained as well as in sectioned material. Furthermore, the annulate lamellae are shown to be a site of activity of the Mg-Na-K-stimulated ATPase.