Quantitative Assessment of Tendon Healing by Using MR T2 Mapping in a Rabbit Achilles Tendon Transection Model Treated with Platelet-rich Plasma
Quantitative Assessment of Tendon Healing by Using MR T2 Mapping in a Rabbit Achilles Tendon Transection Model Treated with Platelet-rich Plasma
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DOI：
10.1148/radiol.2015141544
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发表时间：
2015-09-01
期刊：
影响因子：
19.7
通讯作者：
Takahashi, Kazuhisa
中科院分区：
文献类型：
--
作者：
Fukawa, Taisuke;Yamaguchi, Satoshi;Takahashi, Kazuhisa
目的：为了确定是否磁共振（MR）成像T2映射可以用来量化组织学肌腱愈合，通过使用兔跟腱横断模型处理与富血小板血浆（PRP）。材料和方法：实验机构动物护理和使用委员会批准。手术切断24只新西兰白色家兔（48条肢体）的跟腱，并将PRP（试验组）或生理盐水（对照组）注射到横断部位。术后2、4、8、12周处死动物。此后，使用博纳尔量表进行T2标测和组织学评价。采用混合模型多变量方差分析分别检验时间和PRP治疗对T2值和博纳尔分级的影响。T2值和博纳尔等级之间的相关性也通过斯皮尔曼相关系数进行了评估。结果：在肌腱愈合过程中，两组的博纳尔等级值均下降。T2值也随时间缩短（两组P <0.001）。T2值与博纳尔分级呈正相关（r = 0.78，P <0.001）。PRP组T2值和博纳尔评分在4、8、12周均低于对照组，但PRP治疗对T2值和博纳尔评分无显著影响。虽然PRP处理的肌腱在所有时间点的T2和博纳尔等级均较低，但处理和对照肌腱之间无显著差异。(C)RSNA，2015年
Purpose: To determine if magnetic resonance (MR) imaging T2 mapping can be used to quantify histologic tendon healing by using a rabbit Achilles tendon transection model treated with platelet-rich plasma (PRP).Materials and Methods: Experiments were approved by the Institutional Animal Care and Use Committee. The Achilles tendons of 24 New Zealand white rabbits (48 limbs) were surgically transected, and PRP (in the test group) or saline (in the control group) was injected into the transection site. The rabbits were sacrificed 2, 4, 8, and 12 weeks after surgery. Thereafter, T2 mapping and histologic evaluations were performed by using the Bonar scale. A mixed-model multivariate analysis of variance was used to test the effects of time and PRP treatment on the T2 value and Bonar grade, respectively. The correlation between the T2 value and Bonar grade was also assessed by using the Spearman correlation coefficient.Results: The Bonar scale values decreased in both groups during tendon healing. The T2 value also shortened over time (P < .001 for both groups). The T2 values were positively correlated with the Bonar grade (r = 0.78, P < .001). Both the T2 value and Bonar scale value were lower in the PRP group than in the control group at 4, 8, and 12 weeks; however, there was no significant effect of PRP treatment on the T2 value or Bonar grade.Conclusion: The T2 value changes reflected histologic tendon healing. While T2 and Bonar grade were lower at all time points in tendons treated with PRP, there was no significant difference between the treatment and control tendons. (C) RSNA, 2015