MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms
MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms
复制标题
DOI：
10.1073/pnas.1630797100
复制
发表时间：
2003-08-19
影响因子：
11.1
通讯作者：
Cullen, BR
中科院分区：
文献类型：
--
作者：
Zeng, Y;Yi, R;Cullen, BR
microRNA（miRNAs）是一类内源性编码的非编码小RNA，通过加工短RNA发夹而获得，可抑制含有部分互补靶序列的mRNA的翻译。相比之下，小干扰RNA（siRNA），其通过长双链RNA的加工而衍生并且通常是外源来源的，降解具有完全互补序列的mRNA。在这里，我们证明了内源性编码的人miRNA能够切割具有完全互补的靶位点的mRNA，而外源性提供的siRNA可以抑制具有部分互补序列的mRNA的表达，而不诱导可检测的RNA切割。这些数据表明，miRNA和siRNA可以使用类似的机制来抑制mRNA表达，并且机制的选择可能在很大程度上或完全取决于RNA靶标的互补程度。
MicroRNAs (miRNAs) are endogenously encoded small noncoding RNAs, derived by processing of short RNA hairpins, that can inhibit the translation of mRNAs bearing partially complementary target sequences. In contrast, small interfering RNAs (siRNAs), which are derived by processing of long double-stranded RNAs and are often of exogenous origin, degrade mRNAs bearing fully complementary sequences. Here, we demonstrate that an endogenously encoded human miRNA is able to cleave an mRNA bearing fully complementary target sites, whereas an exogenously supplied siRNA can inhibit the expression of an mRNA bearing partially complementary sequences without inducing detectable RNA cleavage. These data suggest that miRNAs and siRNAs can use similar mechanisms to repress mRNA expression and that the choice of mechanism may be largely or entirely determined by the degree of complementary of the RNA target.