Sensory hyperinnervation after neonatal skin wounding: effect of bupivacaine sciatic nerve block
Sensory hyperinnervation after neonatal skin wounding: effect of bupivacaine sciatic nerve block
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DOI：
10.1093/bja/83.4.662
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发表时间：
1999-10-01
影响因子：
9.8
通讯作者：
Fitzgerald, M
中科院分区：
文献类型：
--
作者：
De Lima, J;Alvares, D;Fitzgerald, M
对组织损伤的反应包括外周伤害感受器和中枢神经元通路的敏化，导致急性临床疼痛和炎性疼痛。进一步的反应是皮肤损伤区域的感觉神经末梢萌芽。与成人相比，新生儿的这种过度神经支配反应尤其强烈。在这项研究中，我们测试了受伤时区域神经阻滞对皮肤过度神经支配的影响。对麻醉的新生大鼠幼崽进行经皮坐骨神经阻滞注射0.25％布比卡因25μl，随后局部后爪皮肤伤口。受伤后7天，通过免疫染色皮肤切片的图像分析来研究皮肤神经支配。并使用冯·普雷毛发评估感觉阈值。结果表明，损伤时应用区域神经阻滞并没有显着改变过度神经支配和超敏反应。这表明临床实践中常用的局部镇痛不太可能预防皮肤受伤后的过度神经支配。
The response to tissue injury includes sensitization of peripheral nociceptors and central neuronal pathways leading to acute clinical and inflammatory pain. A further response is sprouting of sensory nerve terminals in the region of skin damage. This hyperinnervation response is particularly intense in neonates compared with adults. In this study, we tested the effect of regional nerve block at the time of injury on skin hyperinnervation. Anaesthetized newborn rat pups were treated with percutaneous sciatic nerve block injections of 0.25% bupivacaine 25 mu l followed by a localized hindpaw skin wound. Cutaneous innervation was studied by image analysis of immunostained skin sections, 7 days after wounding. and sensory thresholds were assessed using von prey hairs. The results showed that both hyperinnervation and hypersensitivity were not significantly altered by the application of a regional nerve block at the time of injury. This suggests that regional analgesia, used commonly in clinical practice, is unlikely to prevent the hyperinnervation that follows skin wounding.