Genetic diversity of the iron-binding protein (Fbp) gene of the pathogenic and commensal Neisseria.
Genetic diversity of the iron-binding protein (Fbp) gene of the pathogenic and commensal Neisseria.
复制标题
致病性和共生奈瑟氏球菌的铁结合蛋白（Fbp）基因的遗传多样性。
DOI：
10.1111/j.1574-6968.1994.tb06690.x
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发表时间：
1994
影响因子：
2.1
通讯作者：
Trees,DL
中科院分区：
文献类型：
--
作者：
Genco,CA;Berish,SA;Chen,CY;Morse,S;Trees,DL
在铁限制条件下生长时，致病性奈瑟菌和大多数共生奈瑟菌产生铁结合蛋白（Fbp）。在目前的研究中，我们证实了Fbp的存在，以及与淋球菌Fbp同源的DNA序列。淋病奈瑟菌、脑膜炎奈瑟菌、灰霉病奈瑟菌、内酰胺奈瑟菌、亚黄奈瑟菌、克氏奈瑟菌和奈瑟菌。polysaccharea。通过聚合酶链反应扩增这些菌株的fbp基因，用stui或rsai消化，并检查限制性模式。淋球菌感染和脑膜炎球菌感染的模式几乎相同；然而，在寄生线虫种的结果中观察到变化。经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和与Fbp特异性单克隆抗体的反应性检测，黄乳杆菌、N.粘膜、N. sicca、N. ovisand卡他氏branhamella catarrhalis均不产生Fbp，也不能与Fbp特异性DNA探针杂交。
The pathogenicNeisseriaand most commensalNeisseriaspecies produce an iron-binding protein (Fbp) when grown under iron-limited conditions. In the current study, we confirmed the presence of Fbp, as well as DNA sequences homologous to the gonococcalfbp, in strains ofN. gonorrhoeae, N. meningitidis, N. cinerea, N. lactamica, N. subflava, N. kochiiandN. polysaccharea. Thefbpgenes from these strains were amplified by the polymerase chain reaction, digested withStuI orRsaI, and the restriction patterns examined. The patterns for the gonococcal and meningococcalfbpwere virtually identical; however, variations were observed in thefbpsequences of the commensalNeisseriaspecies.N. flavescens, N. mucosa, N. sicca, N. ovisandBranhamella catarrhalis, did not produce Fbp as detected by sodium dodecyl sulfate-polyacrylamide gel electropheris and reactivity with an Fbp specific monoclonal antibody, nor did they hybridize to anfbp-specific DNA probe.