MicroRNA expression profiling defines the impact of electronic cigarettes on human airway epithelial cells.
MicroRNA expression profiling defines the impact of electronic cigarettes on human airway epithelial cells.
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DOI：
10.1038/s41598-017-01167-8
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发表时间：
2017-04-24
影响因子：
4.6
通讯作者：
Mariani TJ
中科院分区：
文献类型：
--
作者：
Solleti SK;Bhattacharya S;Ahmad A;Wang Q;Mereness J;Rangasamy T;Mariani TJ
尽管所有形式的烟草暴露都会对健康产生负面影响，但暴露在电子烟(ECig)中的意义还没有得到充分的了解。在这里，我们研究了eCig对人肺上皮细胞微RNA(MiRNA)转录组的整体影响。将气液界面分化的原代人支气管上皮(NHBE)细胞暴露于eCig液中。NHBE暴露在任何eCig液体中都会导致氧化应激反应基因的诱导，包括GCLM、GCLC、GPX2、NQO1和HO-1。ECig液体中尼古丁的蒸发和/或尼古丁的存在与更大的反应有关。我们在NHBE中发现了578个受eCig暴露影响的miRNAs，以及125个受eCig液体蒸发影响的miRNA。含有eCig蒸气的尼古丁对miRNA表达的影响最大。我们选择了8个miRNAs(29A、140、126、374A、26A-2、147B、941和589)进行进一步研究。我们证实，在eCig暴露后，包括miR126在内的多个miRNAs的表达增加。我们还发现，暴露后两个miR126靶基因MYC和MRGPRX3的表达显著降低。这些数据表明，eCig暴露对人肺上皮细胞的基因表达有深远的影响，其中一些基因是在miRNA调控水平上进行表观遗传编程的。
While all forms of tobacco exposure have negative health effects, the significance of exposure to electronic cigarettes (eCig) is not fully understood. Here, we studied the global effects of eCig on the micro RNA (miRNA) transcriptome in human lung epithelial cells. Primary human bronchial epithelial (NHBE) cells differentiated at air-liquid interface were exposed to eCig liquid. Exposure of NHBE to any eCig liquid resulted in the induction of oxidative stress-response genes including GCLM, GCLC, GPX2, NQO1 and HO-1. Vaporization of, and/or the presence of nicotine in, eCig liquid was associated with a greater response. We identified 578 miRNAs dysregulated by eCig exposure in NHBE, and 125 miRNA affected by vaporization of eCig liquid. Nicotine containing eCig vapor displayed the most profound effects upon miRNA expression. We selected 8 miRNAs (29A, 140, 126, 374A, 26A-2, 147B, 941 and 589) for further study. We validated increased expression of multiple miRNAs, including miR126, following eCig exposure. We also found significant reduction in the expression of two miR126 target genes, MYC and MRGPRX3, following exposure. These data demonstrated that eCig exposure has profound effects upon gene expression in human lung epithelial cells, some of which are epigenetically programmed at the level of miRNA regulation.