Vitamin B6 suppresses NF-kappaB activation in LPS-stimulated mouse macrophages.
Vitamin B6 suppresses NF-kappaB activation in LPS-stimulated mouse macrophages.
复制标题
DOI：
--
复制
发表时间：
2005
影响因子：
5.4
通讯作者：
N. Yanaka;T. Koyama;S. Komatsu;Eri Nakamura;Mayumi Kanda;N. Kato
中科院分区：
文献类型：
--
作者：
N. Yanaka;T. Koyama;S. Komatsu;Eri Nakamura;Mayumi Kanda;N. Kato
虽然维生素B6被认为具有抗炎作用，但其分子机制尚未完全了解。为了探讨维生素B6抗炎作用的机制，我们研究了维生素B6对脂多糖（LPS）刺激的RAW 264.7巨噬细胞炎症反应的影响。本研究表明，维生素B6（吡哆醛）预处理的RAW细胞抑制LPS诱导的iNOS和考克斯-2的表达在mRNA和蛋白质水平。维生素B6抑制LPS诱导的NF-κ B（促炎转录因子）核转位，并降低RAW细胞中LPS诱导的IkappaB α降解程度。虽然维生素B6不影响细胞蛋白酶体活性，但谷胱甘肽S-转移酶融合IkappaB α的体外磷酸化分析表明，维生素B6抑制LPS诱导的IkappaB激酶活化。此外，我们证明，提高饮食中的维生素B6抑制NO的生产在体内响应LPS管理。这些观察结果表明，维生素B6的抗炎作用是通过抑制NF-κ B活化介导的。
Although vitamin B6 has been supposed to have anti-inflammatory effects, the molecular mechanism is not fully understood. To explore the mechanism of anti-inflammatory effects of vitamin B6, we have examined the effect of vitamin B6 on lipopolysaccharide (LPS)-stimulated inflammatory response in RAW 264.7 macrophages. This study demonstrated that vitamin B6 (pyridoxal) pretreatment of RAW cells inhibited LPS-induced expression of iNOS and COX-2 at the mRNA and protein levels. Vitamin B6 inhibited LPS-induced nuclear translocation of the NF-kappaB, the proinflammatory transcription factor, with reduction of the extent of LPS-induced IkappaBalpha degradation in RAW cells. Although vitamin B6 did not affect cellular proteasome activity, in vitro phosphorylation analysis with glutathione S-transferase-fused IkappaBalpha has shown that vitamin B6 suppressed LPS-induced IkappaB kinase activation. Furthermore, we demonstrated that elevating dietary vitamin B6 suppressed NO production in vivo in response to LPS administration. These observations suggest that the anti-inflammatory effect of vitamin B6 is mediated by suppression of NF-kappaB activation.