Center for Synchrotron Biosciences' U2B beamline: an international resource for biological infrared spectroscopy
Center for Synchrotron Biosciences' U2B beamline: an international resource for biological infrared spectroscopy
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DOI：
10.1107/s0909049502008543
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发表时间：
2002-07-01
影响因子：
2.5
通讯作者：
Chance, MR
中科院分区：
文献类型：
--
作者：
Marinkovic, NS;Huang, R;Chance, MR
专门用于生物医学和生物科学的同步加速器红外（IR）光束线U2 B已经建成，并在布鲁克海文国家实验室的国家同步加速器光源（NSLS）中运行。该设施由阿尔伯特·爱因斯坦医学院的同步加速器生物科学中心与国家空间研究所合作运营。由于宽带性质的同步加速器光束的亮度1000倍，传统的来源，傅里叶变换红外光谱实验是可行的衍射有限的样品区域在高信噪比和相对较短的数据采集时间。本文总结了一些在中红外光谱范围（500-5000 cm（-1））内进行的同步辐射红外显微镜实验，包括微秒时间范围内的时间分辨蛋白质折叠研究，神经元、骨和其他生物组织的红外成像，以及化学和环境科学中感兴趣的样品的成像。由于该光束线在远红外区域（50-500 cm（-1））的高通量输出，已经使用定制的低温恒温器和真空箱在10 - 300 K进行了生物分子的氢键和动态分子运动的研究。该设施旨在作为生物红外光谱学的国际资源，根据竞争性提案完全向外部用户提供。
A synchrotron infrared (IR) beamline, U2B, dedicated to the biomedical and biological sciences has been constructed and is in operation at the National Synchrotron Light Source (NSLS) of Brookhaven National Laboratory. The facility is operated by the Center for Synchrotron Biosciences of the Albert Einstein College of Medicine in cooperation with the NSLS. Owing to the broadband nature of the synchrotron beam with brightness 1000 times that of conventional sources, Fourier transform IR spectroscopy experiments are feasible on diffraction-limited sample areas at high signal-to-noise ratios and with relatively short data-acquisition times. A number of synchrotron IR microscopy experiments that have been performed in the mid-IR spectral range (500-5000 cm(-1)) are summarized, including time-resolved protein-folding studies in the microsecond time regime, IR imaging of neurons, bone and other biological tissues, as well as imaging of samples of interest in the chemical and environmental sciences. Owing to the high flux output of this beamline in the far-IR region (50-500 cm(-1)), investigations of hydrogen bonding and dynamic molecular motions of biomolecules have been carried out from 10 to 300 K using a custom-made cryostat and an evacuated box. This facility is intended as an international resource for biological IR spectroscopy fully available to outside users based on competitive proposal.