RECOMBINANT BASIC FIBROBLAST GROWTH-FACTOR ACCELERATES WOUND-HEALING
RECOMBINANT BASIC FIBROBLAST GROWTH-FACTOR ACCELERATES WOUND-HEALING
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DOI：
10.1016/0022-4804(88)90034-0
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发表时间：
1988-07-01
影响因子：
2.2
通讯作者：
DEMETRIOU, AA
中科院分区：
文献类型：
--
作者：
MCGEE, GS;DAVIDSON, JM;DEMETRIOU, AA
碱性成纤维细胞生长因子（bFGF）刺激细胞外基质代谢、生长和中胚层来源细胞的运动。我们以前已经表明，聚乙烯醇海绵中的胶原蛋白含量增加bFGF治疗后。我们假设bFGF治疗的切口伤口愈合更快。戊巴比妥腹腔内麻醉后，雄性200- 250-g Sprague-Dawley大鼠（n= 27）各接受两组成对的横向背部切口，用钢丝缝合。在创伤后第3天，将0.4ml bFGF（重组体，400 ng，Synergen）或生理盐水注射到每对切口中的一个中。在伤后第5、6和7天，用乙醚杀死动物，并切除其背部毛皮。使用张力计对新鲜或福尔马林固定的缠绕条带进行拉伸强度测量。计算了断裂能。使用高效液相色谱法测定水解后伤口边缘样品中的伤口胶原蛋白含量（羟脯氨酸）。bFGF处理的切口的新鲜伤口抗张强度（13.7 ± 1.06 vs 19.1 ± 1.99 g/mm，P< 0.01）和伤口断裂能（476 ± 47 vs 747 ± 76 mm 2，P< 0.001）总体上显著增加。伤口胶原蛋白含量增加，但无统计学显著性，固定切口抗张强度无差异。组织学检查显示bFGF伤口的组织化和成熟程度更好。重组bFGF促进正常大鼠伤口愈合。这可能是由于bFGF治疗的伤口中胶原蛋白和成纤维细胞的早期积累和/或胶原蛋白交联程度更高。
Basic fibroblast growth factor (bFGF) stimulates extracellular matrix metabolism, growth, and movement of mesodermally derived cells. We have previously shown that collagen content in polyvinyl alcohol sponges increased after bFGF treatment. We hypothesized that bFGF-treated incisional wounds would heal more rapidly. After intraperitoneal pentobarbital anesthesia, male, 200- to 250-g, Sprague-Dawley rats (n= 27) each underwent two sets of paired, transverse, dorsal incisions closed with steel sutures. On Day 3 postwounding, 0.4 ml of bFGF (recombinant, 400 ng, Synergen) or normal saline was injected into one of each paired incisions. Animals were killed with ether on postwounding Days 5, 6, and 7 and their dorsal pelts were excised. Fresh or formalin-fixed wound strips were subjected to tensile strength measurements using a tensiometer. Breaking energy was calculated. Wound collagen content (hydroxyproline) was measured in wound-edge samples following hydrolysis using high-performance liquid chromatography. There was an overall significant increase in fresh wound tensile strength (13.7 ± 1.06 vs 19.1 ± 1.99 g/mm,P< 0.01) and wound breaking energy (476 ± 47 vs 747 ± 76 mm2,P< 0.001) in bFGF-treated incisions. There was an increase in wound collagen content which was not statistically significant and there was no difference in fixed incisional tensile strength. Histologic examination showed better organization and maturation in bFGF wounds. Recombinant bFGF accelerates normal rat wound healing. This may be due to earlier accumulation of collagen and fibroblasts and/or to greater collagen crosslinking in bFGF-treated wounds.