Fatty acid amide hydrolase is located preferentially in large neurons in the rat central nervous system as revealed by immunohistochemistry.
Fatty acid amide hydrolase is located preferentially in large neurons in the rat central nervous system as revealed by immunohistochemistry.
复制标题
免疫组织化学显示，脂肪酸酰胺水解酶优先位于大鼠中枢神经系统的大神经元中。
DOI：
10.1016/s0304-3940(98)00700-9
复制
发表时间：
1998
影响因子：
2.5
通讯作者：
Walker,JM
中科院分区：
文献类型：
--
作者：
Tsou,K;Nogueron,MI;Muthian,S;Sañudo-Pena,MC;Hillard,CJ;Deutsch,DG;Walker,JM
用免疫组织化学方法研究了脂肪酸酰胺水解酶(FAAH)在大鼠脑内的分布。在这些研究中使用了针对FAAH C末端区域的免疫纯化的多克隆抗体。大脑皮层的锥体细胞、海马区的锥体细胞、小脑皮质的浦肯野细胞和嗅球的二尖瓣细胞等大型主神经元的FAAH免疫反应最强。我们先前的免疫组织化学研究显示，除嗅球中的二尖瓣细胞外，这些含有FAAH的主神经元与大麻素CB1受体密切相关。在杏仁核、基底节、小脑深核、丘脑腹侧后核、上丘视层和中间白层、中脑红核和脊髓运动神经元中也可见中、淡染色的FAAH神经元。这些数据表明FAAH是不均匀分布的，这种分布与大麻素CB1受体在大鼠脑内的分布有相当大的重叠，尽管不是完全的。
The distribution in the rat brain of fatty acid amide hydrolase (FAAH) an enzyme that catalyzes the hydrolysis of the endogenous cannabinoid anandamide was studied by immunohistochemistry. An immunopurified, polyclonal antibody to the C terminal region of FAAH was used in these studies. The large principal neurons, such as pyramidal cells in the cerebral cortex, the pyramidal cells the hippocampus, Purkinje cells in the cerebellar cortex and the mitral cells in the olfactory bulb, showed the strongest FAAH immunoreactivity. These FAAH-containing principal neurons except the mitral cells in the olfactory bulb are in close proximity with cannabinoid CB1 receptors as revealed by our previous immunohistochemical study. Moderately or lightly stained FAAH-containing neurons were also found in the amygdala, the basal ganglia, the deep cerebellar nuclei, the ventral posterior nuclei of the thalamus, the optic layer and the intermediate white layer of the superior colliculus and the red nucleus in the midbrain, and motor neurons of the spinal cord. These data demonstrate that FAAH is heterogeneously distributed and this distribution exhibits considerable, although not complete, overlap with the distribution of cannabinoid CB1 receptors in rat brain.