THE ORGANIZATION, NUCLEOTIDE-SEQUENCE, AND CHROMOSOMAL DISTRIBUTION OF A SATELLITE DNA FROM ALLIUM-CEPA
THE ORGANIZATION, NUCLEOTIDE-SEQUENCE, AND CHROMOSOMAL DISTRIBUTION OF A SATELLITE DNA FROM ALLIUM-CEPA
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DOI：
10.1007/bf00348692
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发表时间：
1985-01-01
期刊：
影响因子：
1.6
通讯作者：
JAMIESON, G
中科院分区：
文献类型：
--
作者：
BARNES, SR;JAMES, AM;JAMIESON, G
本文报道了一个串联重复的A. cepa（百合科）植物的研究。这颗卫星约占A. cepa基因组，可以从主带DNA中分辨出，并且具有约375个碱基对（bp）的重复长度。重复序列家族的克隆成员仅与染色体端粒杂交，并在间期核中具有非随机分布。呈现了3个重复的核苷酸序列，这些重复在大量位置上不同。除了由375-bp重复序列组成的阵列之外，在具有更大重复长度的单元中发现同源序列。重复序列之间的这种差异反映了使用其他标准确定的卫星的异质性。可能的限制染色体间交换的重复序列进行了讨论。
The organization, nucleitide sequence and chromosomal distribution of a tandemly repeated, satellite DNA from A. cepa (Liliaceae) were studied. The satellite, which constitutes about 4% of the A. cepa genome, may be resolved from main-band DNA in antibiotic-CsCl density gradients, and has a repeat length of about 375 base pairs (bp). A cloned member of the repeat family hybridizes exclusively to chromosome telomeres and has a non-random distribution in interphase nuclei. The nucleotide sequences of 3 repeats, which differ at a large number of positions are presented. In addition to arrays made up of 375-bp repeats, homologous sequence are found in units with a greater repeat length. This divergence between repeats reflects the heterogeneity of the satellite determined using other criteria. Possible constraints on the interchrmosomal exchange of repeated sequences are discussed.