Limonoids from the fruits of Melia azedarach and their cytotoxic activities
Limonoids from the fruits of Melia azedarach and their cytotoxic activities
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DOI：
10.1016/j.phytochem.2013.01.015
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发表时间：
2013-05-01
期刊：
影响因子：
3.8
通讯作者：
Tokuda, Harukuni
中科院分区：
文献类型：
--
作者：
Akihisa, Toshihiro;Pan, Xin;Tokuda, Harukuni
从苦楝科苦楝果实中分离到31个柠檬烯类化合物和1个三萜。通过光谱分析和文献比较，对其中14个化合物的结构进行了鉴定。所有这些化合物都进行了评价。对HL60、A549、AZ521和SK-BR-3人癌细胞的细胞毒活性。Meliarachin C （IC50为0.65 μ M）和3-O-deacetyl-4'-demethyl-28-oxosalannin （IC50为2.8 μ M）对HL60细胞表现出强大的细胞毒活性，这主要是通过流式细胞术诱导细胞凋亡来证明的。Western blot分析表明，这两种化合物通过线粒体和死亡受体介导的途径诱导细胞凋亡。此外，研究了25种化合物对12- o - tetradecanoylphorol -13-acetate （TPA）诱导的Raji细胞eb病毒早期抗原（EBV-EA）活化的抑制作用。(C) 2013 Elsevier Ltd.版权所有。
Thirty-one limonoids and one tirucallane-type triterpenoid were isolated from the fruits of Melia azedarach (Meliaceae). The structures of 14 of these isolated compounds were elucidated on the basis of spectroscopic analyses and comparison with literature. All of these compounds were evaluated for. their cytotoxic activities against HL60, A549, AZ521, and SK-BR-3 human cancer cell lines. Meliarachin C (IC50 0.65 mu M) and 3-O-deacetyl-4'-demethyl-28-oxosalannin (IC50 2.8 mu M) exhibited potent cytotoxic activity against HL60 cells, and this was demonstrated mainly due to the induction of apoptosis by flow cytometry. Western blot analysis suggested that both compounds induced apoptosis via both the mitochondrial and death receptor-mediated pathways. In addition, 25 compounds were evaluated for their inhibitory effects against the Epstein-Barr virus early antigen (EBV-EA) activation induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) in Raji cells. (C) 2013 Elsevier Ltd. All rights reserved.