Apical plasma membrane-bound enzymes of rabbit uterine epithelium
Apical plasma membrane-bound enzymes of rabbit uterine epithelium
复制标题
兔子宫上皮顶端质膜结合酶
DOI：
--
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
E. Winterhager
中科院分区：
文献类型：
--
作者：
I. Classen;H. Denker;E. Winterhager
总结为了监测兔子宫上皮顶端细胞膜的变化（假定为滋养层附着的先决条件），在围着床期研究了标记酶：碱性磷酸酶、氨肽酶M、γ-谷氨酰转移酶和二肽基肽酶IV。早期妊娠子宫内膜（着床室，胚泡间子宫内膜;妊娠后5-8天，d p.c.）与hCG诱导的假孕（p. hCG）中获得的标本进行比较，以区分由母体血浆类固醇激素调节的膜变化和可能由胚泡来源的信号局部诱导的膜变化。p. hCG。这一系列阶段中最弱的反应通常在接种后8天发现。（胚泡间节段）或8 d p. hCG。与其他上皮相比，着床室保留了高活性的二肽基肽酶IV，碱性磷酸酶的活性甚至在交配后7和8天再次升高。表明胚泡对腔上皮的直接局部影响。结果表明：1）当子宫内膜进入“接受状态”时，子宫上皮细胞顶端质膜的组成发生了相当大的变化，2）总体趋势是该膜域的顶端型特征的丧失，3）这些变化受到系统（通过血浆类固醇激素水平）和来自植入胚泡的信号的局部调节。
SummaryIn order to monitor changes in the apical cell membrane of rabbit uterine epithelium which are postulated to be a precondition for trophoblast attachment, the marker enzymes: alkaline phosphatase, aminopeptidase M, γ-glutamyl transferase and dipeptidyl peptidase IV were investigated during the periimplantation phase. Endometrium of early pregnancy (implantation chamber, interblastocyst endometrium; 5–8 days post coitum, d p.c.) was compared with specimens obtained at hCG-induced pseudopregnancy (p. hCG) to distinguish between membrane changes regulated by maternal plasma steroid hormones and such which might be induced locally by blastocyst-derived signals.All enzymes tested showed their main activity at 5 d p.c./p. hCG. The weakest reaction in this series of stages was generally found at 8 d p.c. (interblastocyst segments) or at 8 d p. hCG. In contrast to the rest of the epithelium, the implantation chamber retained high activity of dipeptidyl peptidase IV, and the activity of alkaline phosphatase even raised here again at 7 and 8 d p.c. indicating a direct local influence of the blastocyst on the luminal epithelium. The results suggest that 1) considerable changes occur in the composition of the apical plasma membrane of the uterine epithelium when the endometrium enters the “receptive state”, 2) the overall trend is towards a loss of apical-type characteristics of this membrane domain and 3) the changes are modulated both systemically (by plasma steroid hormone levels) and locally by signals from the implanting blastocyst.
细胞表面糖缀合物的变异性——与细胞功能差异的关系。
DOI：
10.1177/29.9.6169762
发表时间：
1981
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Spicer,SS;Baron,DA;Sato,A;Schulte,BA
通讯作者：
Schulte,BA
在 LLC-PK1 细胞组织成上皮膜的过程中，碱性磷酸酶和 γ-谷氨酰转肽酶作为极化标记。
DOI：
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发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Rabito,CA;Kreisberg,JI;Wight,D
通讯作者：
Wight,D