Histomorphometry and bone biomarkers in cynomolgus females: A study in young, mature, and old monkeys
Histomorphometry and bone biomarkers in cynomolgus females: A study in young, mature, and old monkeys
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DOI：
10.1016/s8756-3282(98)00149-5
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发表时间：
1999-01-01
期刊：
影响因子：
4.1
通讯作者：
Ramsay, H
中科院分区：
文献类型：
--
作者：
Lees, CJ;Ramsay, H
虽然卵巢切除的食蟹猴被广泛用于检查骨骼围绝经期变化的研究，但对这些动物在生长和衰老过程中自然发生的变化知之甚少。为了评价雌性食蟹猴在生长和衰老期间的骨变化，对28只年龄范围为3岁至>22岁的猴给予骨荧光染料标记，并收集髂骨活检。在活检时收集血清和尿液，以测定碱性磷酸酶（ALP）、骨钙素、抗酒石酸酸性磷酸酶（TRAP）、血清和尿钙、雌二醇、尿肌酐和尿CrossLaps水平。对8例年轻（3-3岁）、13例成熟（11-16岁）和8例老年（>22岁）的活检组织进行组织形态计量学分析。与年轻组相比，成熟和老年猴的ALP、TRAP和尿CrossLaps水平较低。尿钙/尿肌酐水平随年龄增长而增加。骨体积（BV/TV）、骨小梁数量（Tb.N）、骨形成率（BFR）和激活频率（Ac.f）在年轻猴中最大，在老年组中下降。幼年动物的生物标志物和组织形态测量指数反映了该组动物的生长情况。年龄较大的猴子在10岁和22岁之间似乎没有显着差异。(C)1999年，Elsevier Science Inc. All rights reserved.
Although ovariectomized cynomolgus monkeys are used extensively for studies examining perimenopausal changes in the skeleton, very little is known al,out the changes that occur naturally during growth and aging in these animals. To evaluate the changes in bone during growth and aging in female cynomolgus monkeys, 28 monkeys ranging from 3 years to >22 years of age were given a bone fluorochrome label and iliac biopsies were collected. Serum and urine were collected at the time of biopsy to determine alkaline phosphatase (ALP), osteocalcin, tartrate-resistant acid phosphatase (TRAP), serum and urinary calcium, estradiol, urinary creatinine, and urinary CrossLaps levels. The biopsies from 8 young (3-3 years of age), 13 mature (11-16 years of age) and 8 old (>22 years of age) were analyzed histomorphometrically. ALP, TRAP, and urinary CrossLaps levels were lower in the mature and old monkeys when compared with the young group. Urinary calcium/urinary creatinine levels increased with age. Bone volume (BV/TV), trabecular number (Tb.N), bone formation rate (BFR), and activation frequency (Ac.f) were greatest in the young monkeys and declined in the older groups. The biomarker and histomorphometric indices in the young animals reflect the growth that was occurring in this group. The older monkeys do not appear to differ significantly between 10 and 22 years of age. (C) 1999 by Elsevier Science Inc. All rights reserved.