Differential Proteomics of Omental and Subcutaneous Adipose Tissue Reflects Their Unalike Biochemical and Metabolic Properties
Differential Proteomics of Omental and Subcutaneous Adipose Tissue Reflects Their Unalike Biochemical and Metabolic Properties
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DOI：
10.1021/pr800942k
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发表时间：
2009-04-01
影响因子：
4.4
通讯作者：
Peral, Belen
中科院分区：
文献类型：
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作者：
Perez-Perez, Rafael;Ortega-Delgado, Francisco J.;Peral, Belen
肥胖在发达国家呈指数级增长，增加了代谢紊乱和心血管疾病的风险，构成了一个公共卫生问题。人类大网膜和皮下脂肪组织在基因表达谱、代谢和生化特性方面的差异已经得到了很好的描述。由于大网膜脂肪组织与胰岛素抵抗、2型糖尿病和心血管疾病的发展密切相关，我们使用二维荧光差异凝胶电泳（2D-DIGE）和质谱（MS）寻找这两个脂肪库中差异表达的蛋白质。在这个分析中，我们发现了43个蛋白，其中一些通过免疫印迹和免疫染色分析得到了验证。结果表明，两种脂肪库的蛋白质组成在组织特异性分子上存在差异，主要与代谢过程有关，如葡萄糖和脂质代谢、脂质转运、蛋白质合成、蛋白质折叠、应激和炎症反应。这表明与皮下脂肪相比，大网膜的代谢活性更高，细胞应激也增加。这些发现为大网膜脂肪在腹部肥胖相关合并症中的作用提供了一些见解。
Obesity is increasing exponentially in developed countries and constitutes a public health problem by enhancing the risk for metabolic disorder and cardiovascular disease. Differences in gene expression profiles and in metabolic and biochemical properties have been well-described between omental and subcutaneous adipose tissue in humans. Because omental adipose tissue has been strongly associated with the development of insulin resistance, type 2 diabetes and cardiovascular disease, we searched for proteins differentially expressed in these two fat depots using two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) and mass spectrometry (MS). In this analysis, we found 43 proteins, several of which were validated by immunoblotting and immunostaining analyses. Results demonstrated tissue-specific molecular differences in the protein makeup of the two analyzed fat depots mainly related to metabolic processes such as glucose and lipid metabolism, lipid transport, protein synthesis, protein folding, response to stress and inflammation. This suggests higher metabolic activity as well as increased cell stress in the omental compared to the subcutaneous fat. These findings provide some insights into the role of omental fat in abdominal obesity-associated co-morbidities.