Inactivation of feline calicivirus, a surrogate of norovirus (formerly Norwalk-like viruses), by different types of alcohol in vitro and in vivo
Inactivation of feline calicivirus, a surrogate of norovirus (formerly Norwalk-like viruses), by different types of alcohol in vitro and in vivo
复制标题
DOI：
10.1016/j.jhin.2003.12.030
复制
发表时间：
2004-01-01
影响因子：
6.9
通讯作者：
Goroncy-Bermes, P
中科院分区：
文献类型：
--
作者：
Gehrke, C;Steinmann, J;Goroncy-Bermes, P
手消毒是防止诺沃克病毒(以前称为诺沃克病毒)从手或环境表面传播到其他物体的重要措施。因此，研究了三种乙醇(乙醇、正丙醇和异丙醇)作为诺如病毒替代品对猫杯状病毒(FCV)的灭活特性。试验以定量悬浮法或人工污染指尖的活体实验进行。体外实验表明，正丙醇对FCV的灭活效果优于乙醇和异丙醇：在50%和70%的不同醇类溶液中，正丙醇作用30s后可使FCV的灭活效果提高10(4)倍，而其他醇类仅作用3min。在最高浓度(80%)时，效果不是最好的。在悬液试验中，以下浓度(外推数据)对病毒的灭活效果最好：乙醇67%，异丙醇58%(作用时间1min)，正丙醇60%(作用时间30 S)。用70%和90%的乙醇溶液进行指尖试验，涂抹时间为30S，结果证实了这些发现：70%的酒精溶液比90%的溶液更有效。与悬浮试验相比，70%乙醇在体内的效果最好，其LOGO降低系数(Rf)为3.78，而70%正丙醇(Rf3.58)、70%异丙醇(Rf2.15)和硬水(Rf1.23)的效果最差。因此，酒精含量较高的乙醇和正丙醇溶液似乎最有效。(C)2003年医院感染学会。爱思唯尔有限公司出版。保留所有权利。
Hand disinfection is an important measure to prevent transmission of norovirus (formerly called Norwalk-like viruses) from hands or environmental surfaces to other objects. Therefore, three types of alcohol (ethanol, 1- and 2-propanol) were examined for their virus-inactivating properties against feline calicivirus (FCV) as a surrogate for norovirus. Tests were performed as quantitative suspension assays or as in vivo experiments with artificially contaminated fingertips. The in vitro experiments showed that 1-propanol was more effective than ethanol and 2-propanol for the inactivation of FCV: in tests with the 50 and 70% solutions of the different alcohols, a 10(4)-fold reduction was observed with 1-propanol after 30s, whereas the other alcohols were effective only after 3 min contact time. The greatest efficacy did not occur at the highest concentrations (80%). The following concentrations (extrapolated data) showed the greatest virus-inactivating properties in the suspension test: ethanol 67%, 2-propanol 58% (exposure times of 1 min) and 1-propanol 60% (exposure time of 30 s). The results from fingertips experiments with 70 and 90% solutions and an application time of 30 s confirmed these findings: the 70% alcoholic solutions were more effective than the 90% solutions. In contrast to the suspension tests, 70% ethanol showed the greatest efficacy in vivo with a logo reduction factor (RF) of 3.78 compared with 70% 1-propanol (RF 3.58), 70% 2-propanol (RF 2.15) and hard water (RF 1.23). Ethanol and 1-propanol-based solutions with a high alcohol content thus appear most effective. (C) 2003 The Hospital Infection Society. Published by Elsevier Ltd. All rights reserved.