Multilocus sequence typing scheme for Enterococcus faecium
Multilocus sequence typing scheme for Enterococcus faecium
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DOI：
10.1128/jcm.40.6.1963-1971.2002
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发表时间：
2002-06-01
影响因子：
9.4
通讯作者：
Willems, RJL
中科院分区：
文献类型：
--
作者：
Homan, WL;Tribe, D;Willems, RJL
已经针对屎肠球菌开发了多位点序列分型（MLST）方案。分析了来自123个流行病学上与人类和牲畜无关的分离株以及来自美国、英国、澳大利亚和荷兰几次疫情的16个人源分离株的7个管家基因的内部片段。在万古霉素敏感的大肠杆菌中共检测到62种序列类型。屎肠杆菌（VSEF）和耐万古霉素大肠杆菌。屎肠球菌（VREF）分离株。VSEF分离株的遗传多样性高于VREF分离株。VSEF和VREF分离株在宿主特异性谱系中聚类，这与通过扩增片段长度多态性分析获得的宿主特异性聚类相似。从住院病人中分离的暴发菌株聚集在一个亚群中，该亚群由管家基因purK和表面蛋白基因esp.的独特等位基因的存在所定义。faecium最近在世界范围内出现，而VREF和VSEF的遗传变异是通过长期重组产生的。结果表明，E.粪菌提供了一个很好的工具，分离特征和长期的流行病学分析。
A multilocus sequence typing (MLST) scheme has been developed for Enterococcus faecium. Internal fragments from seven housekeeping genes of 123 epidemiologically unlinked isolates from humans and livestock and 16 human-derived isolates from several outbreaks in the United States, the United Kingdom, Australia, and The Netherlands were analyzed. A total of 62 sequence types were detected in vancomycin-sensitive E. faecium (VSEF) and vancomycin-resistant E. faecium (VREF) isolates. VSEF isolates were genetically more diverse than VREF isolates. Both VSEF and VREF isolates clustered in host-specific lineages that were similar to the host-specific clustering obtained by amplified fragment length polymorphism analysis. Outbreak isolates from hospitalized humans clustered in a subgroup that was defined by the presence of a unique allele from the housekeeping gene purK and the surface protein gene esp. The MLST results suggest that epidemic lineages of E. faecium emerged recently worldwide, while genetic variation in both VREF and VSEF was created by longer-term recombination. The results show that MLST of E. faecium provides an excellent tool for isolate characterization and long-term epidemiologic analysis.