A new Augmin subunit, Msd1, demonstrates the importance of mitotic spindle-templated microtubule nucleation in the absence of functioning centrosomes
A new Augmin subunit, Msd1, demonstrates the importance of mitotic spindle-templated microtubule nucleation in the absence of functioning centrosomes
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DOI：
10.1101/gad.532209
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发表时间：
2009-08-15
影响因子：
10.5
通讯作者：
Wakefield, James G.
中科院分区：
文献类型：
--
作者：
Wainman, Alan;Buster, Daniel W.;Wakefield, James G.
果蝇的Augmin复合物将γ -微管蛋白定位于有丝分裂纺锤体的微管，调节组织培养细胞中纺锤体微管的密度。在这里，我们确定了微管相关蛋白Msd1作为Augmin复合物的新组分，并直接证明了它是有丝分裂纺锤体内微管成核所必需的。虽然Msd1对于胚胎合胞有丝分裂是必需的，但是携带Msd1突变的果蝇是可以存活的。然而，重要的是，在中心体缺失的情况下，纺锤体内部的微管成核变得至关重要。因此，Augmin复合物在果蝇的发育中起着至关重要的作用。
The Drosophila Augmin complex localizes gamma-tubulin to the microtubules of the mitotic spindle, regulating the density of spindle microtubules in tissue culture cells. Here, we identify the microtubule-associated protein Msd1 as a new component of the Augmin complex and demonstrate directly that it is required for nucleation of microtubules from within the mitotic spindle. Although Msd1 is necessary for embryonic syncytial mitoses, flies possessing a mutation in msd1 are viable. Importantly, however, in the absence of centrosomes, microtubule nucleation from within the spindle becomes essential. Thus, the Augmin complex has a crucial role in the development of the fly.