Embryo culture media and neonatal birthweight following IVF
Embryo culture media and neonatal birthweight following IVF
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DOI：
10.1093/humrep/der381
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发表时间：
2012-02-01
影响因子：
6.1
通讯作者：
Racowsky, C.
中科院分区：
文献类型：
--
作者：
Eaton, J. L.;Lieberman, E. S.;Racowsky, C.
背景：通过体外受精受孕的婴儿出生体重过低的风险增加。动物研究表明，胚胎培养基会影响新生儿体重，但目前尚不清楚这种关联是否存在于人类身上。方法：我们确定了1999年1月1日至2008年12月31日期间所有开始日期为1999年1月1日至2008年12月31日的体外受精周期，这些周期使用的是自体卵母细胞，所产生的胚胎在G1.3、Global或G1.5培养液中培养。在胎龄为34周的第三天，新鲜的单胚胎移植后的单胎分娩，或第三天的新鲜双胚胎移植后的双胞胎分娩。每名妇女只接受了研究期间的第一个周期。如果超声检查的孕囊数量与出生的婴儿数量不同，女性就被排除在外。变量用卡方检验或方差分析进行评估。结果：在198例单胎分娩的妇女中，102个胚胎在G1.3培养，53个在Global培养，43个在G1.5培养。在303对双胞胎中，172对胚胎在G1.3培养，58对在Global培养，73对在G1.5培养。即使在控制潜在的混杂因素的情况下，也没有观察到培养液和出生体重之间的显著关联。结论：这项回顾性研究显示，体外受精后胚胎培养液和出生体重之间没有显著的关联。虽然我们对患者的仔细选择将潜在混杂因素的影响降到了最低，但还需要进一步的研究来阐明这个问题，因为有更多的患者。
BACKGROUND: Infants conceived from IVF are at increased risk for low birthweight. Animal studies suggest that embryo culture medium influences birthweight but it is unknown whether this association exists in humans. This study examines the relationship between culture medium and birthweight following IVF.METHODS: We identified all IVF cycles with start dates between 1 January 1999 and 31 December 2008 that used autologous oocytes with resulting embryos cultured in G1.3, Global or G1.5 medium. The population was restricted to singleton deliveries following Day 3, fresh single embryo transfer, or twin deliveries following Day 3, fresh double embryo transfer, at a gestational age of >= 34 weeks. Only the first cycle during the study period was included for each woman. Women were excluded if the number of gestational sacs on ultrasound differed from the number of infants born. Variables were evaluated with the chi(2)-test or analysis of variance. Multiple linear regressions controlled for potential confounders.RESULTS: Of the 198 women with singleton deliveries, 102 embryos were cultured in G1.3, 53 in Global and 43 in G1.5 medium. Of the 303 twin deliveries, 172 pairs of embryos were cultured in G1.3, 58 in Global and 73 in G1.5 medium. No significant association between culture medium and birthweight was observed, even when controlling for potential confounders.CONCLUSIONS: This retrospective study demonstrated no significant association between embryo culture medium and birthweight following IVF. Although our careful selection of patients minimized the influence of potential confounders, further research is required to elucidate this issue with larger numbers of patients.