Toxoplasma gondii: Evidence for the transmission by semen in dogs
Toxoplasma gondii: Evidence for the transmission by semen in dogs
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DOI：
10.1016/j.exppara.2009.07.003
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发表时间：
2009-10-01
影响因子：
2.1
通讯作者：
da Costa, Alvimar Jose
中科院分区：
文献类型：
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作者：
Arantes, Tiago Pereira;Zanetti Lopes, Welber Daniel;da Costa, Alvimar Jose
将10只公犬随机分为3组：GI组3只接种2.0×10(5)P菌株卵囊，GII组3只感染1.0×10(6)RH株速殖子，GIII-4组为对照组。对几个临床参数进行评估。IFAT检测抗-T。弓形虫抗体。精液中寄生虫的存在通过聚合酶链式反应和生物测定技术进行评估。用生物测定和免疫组织化学方法检测睾丸和附睾切除后的组织寄生情况。在这两组收集的精液样本中，通过生物检测和聚合酶链式反应证实了弓形虫的存在。用免疫组织化学方法在所有6只实验感染犬的组织(睾丸和附睾片)中检测到弓形虫。用弓形虫阳性精液对4只未感染弓形虫的母犬进行人工授精(AI)。AI后7天，所有雌犬均出现血清学转换(IFAT)。其中两只狗发生了胎儿重吸收，而其余的则持续了足月妊娠。在四个后代的大脑中发现了几个弓形虫包囊。这些结果表明弓形虫可以在家犬中通过性传播。(C)2009 Elsevier Inc.保留所有权利。
Ten male dogs were distributed into three experimental groups for infection with Toxoplasma gondii: GI three dogs inoculated with 2.0 x 10(5) P strais oocysts, GII - three dogs infected with 1.0 x 10(6) RH strain tachyzoites, and GIII - four controls dogs. Several clinical parameters were evaluated. IFAT was performed to detect anti-T. gondii antibodies. Presence of the parasite in semen was evaluated by PCR and bioassay techniques. Tissue parasitism was examined using bioassays and immunohistochemistry in testicle and epididymis fragments collected after orchiectomy. In semen samples collected from these two groups, the presence of T. gondii was verified by bioassays and PCR. T. gondii was detected by immunohistochemistry in tissues (testicle and epididymis fragments) of all six experimentally infected dogs. The T. gondii-positive seminal samples were used in the artificial insemination (AI) of four female dogs free of toxoplasmic infection. Seven days after AI, all of the female dogs presented serologic conversion (IFAT). Fetal reabsorption occurred in two of the dogs, while the others sustained full-term gestation. Several T. gondii cysts were detected in the brains of four offspring. These results suggest that T. gondii can be sexually transmitted in domestic dogs. (C) 2009 Elsevier Inc. All rights reserved.