Enduring pathogenicity of African strains of Salmonella on plastics and glass in simulated peri-urban environmental waste piles
Enduring pathogenicity of African strains of Salmonella on plastics and glass in simulated peri-urban environmental waste piles
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DOI：
10.1016/j.jhazmat.2023.132439
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发表时间：
2023-09-19
影响因子：
13.6
通讯作者：
Quilliam，Richard S.
中科院分区：
文献类型：
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作者：
Ormsby，Michael J.;White，Hannah L.;Quilliam，Richard S.
在低收入和中等收入国家，塑料已经成为垃圾填埋场和城市垃圾场的主要组成部分。环境塑料污染也为微生物生物膜的形成提供了新的表面，微生物生物膜通常包括病原菌和病毒。在这里，在模拟非洲非正式聚居地典型的城市周围垃圾堆的条件下，我们的目标是确定致病因素是否与沙门氏菌有关。在环境塑料和玻璃表面长时间干燥后，可以保持其毒性。我们表明，临床(和环境)相关的沙门氏菌菌株，包括肠炎沙门氏菌、鼠伤寒沙门氏菌和伤寒沙门氏菌，可以在塑料和玻璃上存活至少28天，温度(随着城市垃圾堆的深度增加)是这种存活的关键决定因素。所有三株沙门氏菌在从塑料圈恢复后都保持了它们的致病性(通过使用梅隆格氏杆菌感染模型来确定)，这表明环境中的塑料可以作为人类病原体的储存库，并可以促进它们在较长一段时间内持续存在。因此，环境塑料垃圾中的病原体构成了更高的公共健康风险，特别是在人们经常暴露在塑料污染中的地区。
In low- and middle-income countries, plastic has become a major constituent of landfills and urban dump sites. Environmental plastic pollution can also provide a novel surface for the formation of microbial biofilm, which often includes pathogenic bacteria and viruses. Here, under conditions simulating a peri-urban waste pile typical of an African informal settlement, we aimed to determine if pathogenicSalmonellaspp. can retain their virulence following a prolonged period of desiccation on the surfaces of environmental plastic and glass. We show that clinically (and environmentally) relevant strains ofSalmonellaincludingS.Enteritidis,S.Typhimurium andS.Typhi can persist on plastic and glass for at least 28-days and that temperature (which increases with the depth of an urban waste pile) is a key determinant of this survival. All three strains ofSalmonellaretained their pathogenicity (determined by using aGalleria mellonellamodel of infection) following their recovery from the plastisphere indicating that plastics in the environment can act as reservoirs for human pathogens and could facilitate their persistence for extended periods of time. Pathogens colonising environmental plastic waste therefore pose a heightened public health risk, particularly in areas where people are frequently exposed to plastic pollution.