C-ring cleavage metabolites of catechin and epicatechin enhanced antioxidant activities through intestinal microbiota
C-ring cleavage metabolites of catechin and epicatechin enhanced antioxidant activities through intestinal microbiota
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儿茶素和表儿茶素的 C 环裂解代谢物通过肠道微生物群增强抗氧化活性
DOI：
10.1016/j.foodres.2020.109271
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发表时间：
2020-09-01
影响因子：
8.1
通讯作者：
Liu, Rui
中科院分区：
文献类型：
--
作者：
Chen, Wanbing;Zhu, Xiaoling;Liu, Rui
儿茶素和表儿茶素与粪便微生物体外培养24 h过程中DPPH自由基清除能力的变化以及UPLC-Q-TOF对特征代谢物的针对性分析表明，抗氧化活性的增加与C环裂解代谢物的积累是同步的。因此，C环裂解代谢物1-(3',4'-二羟基苯基)-3-(2'',4'',6''-三羟基苯基)丙-2-醇(3,4-DHPP-2-ol)从培养液中分离出来。通过DPPH和ABTS自由基清除能力和铁还原抗氧化能力（FRAC）检查该代谢物和其他11种代谢物的抗氧化活性。结果表明，所有具有3',4'-二羟基化结构的代谢物均具有较高的抗氧化活性，尤其是3,4-DHPP-2-ol，DPPH测定其EC50为5.97 μM，是儿茶素的2倍，表儿茶素的1.8倍。但苯环上具有单羟基或非羟基结构的代谢物几乎不表现出抗氧化活性。
The changes in DPPH radical-scavenging capability of catechin and epicatechin during 24 h incubation with fecal microbiota in vitro and the targeted analysis o f the characteristic metabolites by using UPLC-Q-TOF indicated that increase in antioxidant activity was synchronous with the accumulation of C-ring cleavage metabolites. Therefore, C-ring cleavage metabolite, 1-(3',4'-Dihydroxyphenyl)-3-(2 '',4 '',6 ''-trihydroxyphenyl)propan-2-ol (3,4-DHPP-2-ol), was separated from incubation liquid. The antioxidant activities of this metabolite and other 11 metabolites were examined through DPPH and ABTS free radical scavenging capacity and ferric reducing antioxidant capability (FRAC). The results indicated that all metabolites with the structure of 3',4'-dihydroxylated had high antioxidant activity, especially 3,4-DHPP-2-ol, whose EC50 was 5.97 mu M in DPPH assay, 2 times as high as that of catechin, and 1.8 times as high as that of epicatechin. But the metabolites with the structure of monohydroxylated or unhydroxylated on the benzene ring hardly exhibited antioxidant activity.