METAL-IONS INDUCE EXPRESSION OF METALLOTHIONEIN IN PANCREATIC EXOCRINE AND ENDOCRINE-CELLS
METAL-IONS INDUCE EXPRESSION OF METALLOTHIONEIN IN PANCREATIC EXOCRINE AND ENDOCRINE-CELLS
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DOI：
10.1097/00006676-199009000-00009
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发表时间：
1990-09-01
期刊：
影响因子：
2.9
通讯作者：
SARRAS, MP
中科院分区：
文献类型：
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作者：
ANDREWS, GK;KAGE, K;SARRAS, MP
北方印迹杂交证实，镉或锌盐可使大鼠胰腺金属硫蛋白（MT）mRNA水平显著升高。为了确定哪种胰腺细胞类型表达MT基因，使用了从富含腺泡的制剂中提取的RNA的北方印迹杂交分析、原位杂交和免疫细胞化学。锌处理的大鼠胰腺腺泡RNA中含有高水平的MT mRNA。在对照组大鼠中，原位杂交表明在胰腺的外分泌和内分泌细胞中MT mRNA的水平非常低，但这些水平显着增加，在这两个细胞群后金属注射。与此相反，胰岛素-I的mRNA水平的内分泌细胞没有受到金属注射。用北方杂交和原位杂交技术检测小鼠和鸡胰腺MT mRNA，结果与此相似。免疫细胞化学检测到MT在大鼠腺泡细胞胞浆中。虽然不一致的原位杂交研究和免疫细胞化学分析外分泌细胞，MT的免疫细胞化学表明一个统一的染色模式的胰岛细胞，不受金属处理。这些结果表明，在胰腺MT基因的金属离子诱导发生在内分泌和外分泌细胞，这表明，这种蛋白质在这个器官有不同的生理功能。
Northern blot hybridization established that metallothionein (MT) mRNA levels were dramatically elevated in the rat pancreas folloiwng injection of Cd or Zn salts. To determine which pancreatic cell types express the MT gene, Northern blot hybridization analysis of RNA from preparations enriched for acini, in situ hybridization, and immunocytochemistry were used. RNA from pancreatic acini of Zn-treated rats contained high levels of MT mRNA. In control rats, in situ hybridization suggested very low levels of MT mRNA in both exocrine and endocrine cells in the pancreas, but these levels were dramatically increased in both these cell populations following metal injection. In contrast, levels of insulin-I mRNA in the endocrine cells were not affected by metal injection. A similar result with MT mRNA was obtained in mouse and chicken pancreas using Northern blot and in situ hybridization. Immunocytochemisty detected MT in the rat acinar cell cytoplasm following metal injection. Although inconsistent with in situ hybridization studies and immunocytochemical analysis of exocrine cells, immunocytochemisty for MT indicated a uniform staining pattern of islet cells that was unaffected by metal treatment. These results establish that metal ion induction of the MT genes in pancreas occurs in both endocrine and exocrine cells, which suggests that this protein has diverse physiologic functions in this organ.