Suppressors of the ndc10-2 mutation: a role for the ubiquitin system in Saccharomyces cerevisiae kinetochore function.
Suppressors of the ndc10-2 mutation: a role for the ubiquitin system in Saccharomyces cerevisiae kinetochore function.
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ndc10-2 突变的抑制剂：泛素系统在酿酒酵母动粒功能中的作用。
DOI：
10.1093/genetics/147.2.409
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发表时间：
1997
期刊：
影响因子：
3.3
通讯作者：
Huffaker,TC
中科院分区：
文献类型：
--
作者：
Kopski,KM;Huffaker,TC
我们在编码酿酒酵母CBF3动粒复合体110-kD亚基的NDC10/CBF2/CTF14基因中分离到一个新的条件致死突变ndc10-2。在37℃的限制性温度下，NDC10-2细胞能够组装后期纺锤体，但不能分离它们的DNA，这与动粒功能的缺陷一致。为了确定在动粒组装或功能中起作用的其他因素，我们分离了Endc10-2突变的剂量和第二位点抑制物。这些筛查确定UBC6为剂量抑制因子，UBC6和UBC7突变为NDC10-2热敏感性的第二位点抑制因子。UBC6和UBC7都编码泛素结合酶，这些酶在泛素介导的蛋白质降解中发挥作用。此外，突变泛素的过表达抑制了Endc10-2突变。这些结果表明泛素系统参与了NDC10-2功能的调节，并提示了泛素系统在动粒功能中的作用。
We have isolated a new conditional-lethal mutation,ndc10-2, in theNDC10/CBF2/CTF14gene that encodes the 110-kD subunit of theSaccharomyces cerevisiaeCBF3 kinetochore complex. At the restrictive temperature of 37°,ndc10-2cells are able to assemble anaphase spindles, but fail to segregate their DNA, consistent with a defect in kinetochore function. To identify other factors that play a role in kinetochore assembly or function, we isolated both dosage and second site suppressors of thendc10-2mutation. These screens identifiedUBC6as a dosage suppressor, and mutations inUBC6andUBC7as second-site suppressors ofndc10-2heat sensitivity. BothUBC6andUBC7encode ubiquitin-conjugating enzymes that function in ubiquitin-mediated protein degradation. Furthermore, overexpression of a mutant ubiquitin suppresses thendc10-2mutation. These results implicate the ubiquitin system in the regulation ofndc10-2function and suggest a role for the ubiquitin system in kinetochore function.