Brain response to cecal infection with Campylobacter jejuni:: analysis with Fos immunohistochemistry
Brain response to cecal infection with Campylobacter jejuni:: analysis with Fos immunohistochemistry
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DOI：
10.1016/j.bbi.2003.08.002
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发表时间：
2004-05-01
影响因子：
15.1
通讯作者：
Lyte, M
中科院分区：
文献类型：
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作者：
Gaykema, RPA;Goehler, LE;Lyte, M
细菌性病原体感染可诱导啮齿动物增加焦虑样行为，而不会出现其他明显的行为或生理疾病症状，食源性病原体空肠弯曲杆菌就是一个例子。这一观察结果暗示了大脑感知并对这种感染做出反应的能力。我们验证了我们的假设，即肠道感染革兰氏阴性细菌空肠c导致处理胃肠道感觉信息的某些大脑区域激活。在口服活C空肠的小鼠大脑中，与盐水处理的对照组相比，评估了C - fos蛋白作为神经元激活标记物的诱导。在肠内定植后，空肠梭菌在口腔攻击后1天和2天激活脑干的内脏感觉核（孤立束核和外侧臂旁核）。此外，第2天下丘脑室旁核c-Fos表达增加。这种神经反应发生在没有可测量的全身免疫激活的情况下，因为血清中肿瘤坏死因子- α、白细胞介素-1 β和白细胞介素-6的水平无法检测到和/或保持不变。这些发现支持了一种观点，即肠道中空肠C菌感染的信息确实传递给了大脑中的内脏感觉结构。观察到的大脑反应可能有助于感染后观察到的行为变化。(C) 2003 Elsevier Inc.版权所有。
Infections with bacterial pathogens can induce increased anxiety-like behaviors in rodents without otherwise noticeable behavioral or physiological symptoms of sickness, as shown with the food-borne pathogen Campylobacter jejuni. This observation implicates the ability of the brain to sense, and respond to, such an infection. We tested our hypothesis that intestinal infection with the gram-negative bacterium C. jejuni leads to activation of certain brain regions that process gastro-intestinal sensory information. The induction of c-Fos protein as a marker for neuronal activation was assessed in the brains of mice inoculated orally with live C jejuni, as compared to saline-treated controls. Upon colonization of the intestines, C. jejuni activated visceral sensory nuclei in the brainstem (the nucleus of the solitary tract and the lateral parabrachial nucleus) both one and two days after the oral challenge. In addition, increased c-Fos expression occurred in the hypothalamic paraventricular nucleus on the second day. This neural response occurred in the absence of measurable systemic immune activation, as serum levels of tumor necrosis factor-alpha, interleukin-1beta, and interleukin-6 were undetectable and/or unchanged. These findings support the notion that information about infection with C jejuni in the gut is indeed relayed to the visceral sensory structures in the brain. The brain responses observed could contribute to changes in behavior observed after infection. (C) 2003 Elsevier Inc. All rights reserved.