Serum paraoxonase activity decreases in rheumatoid arthritis
Serum paraoxonase activity decreases in rheumatoid arthritis
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DOI：
10.1016/s0024-3205(03)00195-4
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发表时间：
2003-05-09
期刊：
影响因子：
6.1
通讯作者：
Hashimoto, K
中科院分区：
文献类型：
--
作者：
Tanimoto, N;Kumon, Y;Hashimoto, K
目的：评估类风湿性关节炎（RA）中对氧磷酶 1（PON-1）和高密度脂蛋白（HDL）的变化。设计和方法：我们研究了 RA 患者的血清酶活性和 PON1 浓度及其与血脂、高密度脂蛋白 (HDL) 参数以及血清淀粉样蛋白 A (SAA) 和 C 反应蛋白 (CRP) 急性期反应物的关系。结果：与健康受试者 (n = 155, 164 59) 相比，RA 患者 (n = 64, 131 +/- 53 mumol/min/L) 的血清对氧磷酶 (PON) 活性显着降低，尽管两组之间的血清脂质水平没有任何差异。在 192 Q/R 的 PON1 的每个基因型（Q/Q、Q/R、R/R）中都发现了 RA 患者血清 PON 活性的降低。 RA 患者和健康受试者之间的 PON1 Q/R 基因型分布不同，RA 患者的 PON1-Q 阳性阳性率 (44%) 低于健康受试者 (66%)。在对年龄和性别匹配的 RA 亚组 (n = 25) 和健康受试者 (n = 25) 的进一步调查中，发现 RA 患者的血清 PON 活性和卵磷脂胆固醇酰基转移酶 (LCAT) 均比健康受试者显着降低 (125 +/- 61 mumol/min/L, 63.2 +/- 17.2 nmol/ml/hr/37°C)分别为（169 +/- 67、74.7 +/- 19.5）。 RA 患者中 PON1 和 LCAT 以及 HDL 成分载脂蛋白 (apo) AI 和 apo All 均发生显着改变。结论：RA 中的急性期 HDL 在结构和功能上进行了重塑，由于原始 HDL 功能受损，其抗动脉粥样硬化能力可能较差。 RA 患者 HDL 的这些变化可能部分解释了所报道的 RA 患者心血管死亡率增加的原因。 (C) 2003 Elsevier Science Inc. 保留所有权利。
Objective: To estimate the alterations of paraoxonase 1 (PON-1) and high-density lipoprotein (HDL) in rheumatoid arthritis (RA). Design and Methods: We investigated the serum enzyme activity and concentration of PON1 and their relationship With serum lipids, high-density lipoprotein (HDL) parameters, and acute phase reactants of serum amyloid A (SAA) and C-reactive protein (CRP) in patients with RA. Results: Serum paraoxonase (PON) activity was significantly decreased in RA patients (n = 64, 131 +/- 53 mumol/min/L) compared with healthy subjects (n = 155, 164 59) despite the absence of any difference in serum lipid levels between the two groups. This decrease of serum PON activity in RA patients was found in every genotype (Q/Q, Q/R, R/R) of PON1 at 192 Q/R. There was a different distribution in PON1 Q/R genotypes between RA patients and healthy subjects, and RA patients exhibited less (44%) positive PON1-Q than did the healthy subjects (66%). In a further investigation of age- and gender-matched subgroups of RA (n = 25) and healthy subjects (n = 25), not only serum PON activity, but also lecithin-cholesterol acyltransferase (LCAT) was found to be significantly decreased in RA patients (125 +/- 61 mumol/min/L, 63.2 +/- 17.2 nmol/ml/hr/37degreesC) than in healthy subjects (169 +/- 67, 74.7 +/- 19.5), respectively. PON1 and LCAT as well as HDL constituent apolipoprotein (apo) AI and apo All, were altered significantly in RA patients. Conclusions: Acute-phase HDL, which is remodeled structurally and functionally in RA, might be less anti-atherogenic due to the impairment of original HDL function. These alterations of HDL in RA patients may explain in part the reported increase in cardiovascular mortality in patients with RA. (C) 2003 Elsevier Science Inc. All rights reserved.