Leucocin C-607, a Novel Bacteriocin from the Multiple-Bacteriocin-Producing Leuconostoc pseudomesenteroides 607 Isolated from Persimmon
Leucocin C-607, a Novel Bacteriocin from the Multiple-Bacteriocin-Producing Leuconostoc pseudomesenteroides 607 Isolated from Persimmon
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DOI：
10.1007/s12602-017-9359-6
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发表时间：
2018-06-01
影响因子：
4.9
通讯作者：
Yanagida, Fujitoshi
中科院分区：
文献类型：
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作者：
Chen, Yi-sheng;Wu, Hui-chung;Yanagida, Fujitoshi
从柿果中分离得到的类肠明串珠菌607对单核细胞增生李斯特菌等革兰氏阳性菌有较强的抑制作用。通过离子交换色谱、Sep-Pak C-18柱和反相高效液相色谱（RP-HPLC）从培养上清液中纯化抑制物质。在纯化过程中观察到两种抗菌肽。这些肽中的一个具有4623.05 Da的分子大小和NH 2-KNYGNGVHxTKKGxS的部分N-末端氨基酸序列，其中YGNGV基序对IIa类细菌素具有特异性。BLAST检索显示，该细菌素与肠膜明串珠菌的白细胞素C相似。设计Leucocin C特异性引物，扩增单一PCR产物。对核苷酸序列的分析揭示了一种推定的肽，其与白细胞菌素C的序列仅相差一个氨基酸残基。在文献中没有报道相同的肽或蛋白质，因此认为该肽称为leucocin C-607，是由Leuc产生的leucocin C的新变体。607.第二次世界大战从同一培养上清中纯化的另一种抗菌肽的分子大小为3007.7或3121.97 Da，但关于该第二种肽的详细信息仍有待确定。两种细菌素具有明显的热稳定性和抑菌谱。607.第二次世界大战这些结果表明，Leuc.肠状假单胞菌607产生白细胞菌素C-607沿着另一种未知的细菌素。
Leuconostoc pseudomesenteroides 607, isolated from persimmon fruit, was found to have high inhibitory activity against Listeria monocytogenes and several other Gram-positive bacteria. Inhibitory substances were purified from culture supernatant by ion-exchange chromatography, Sep-Pak C-18 cartridge, and reverse-phase high-performance liquid chromatography (RP-HPLC). Two antibacterial peptides were observed during the purification procedures. One of these peptides had a molecular size of 4623.05 Da and a partial N-terminal amino acid sequence of NH2-KNYGNGVHxTKKGxS, in which the YGNGV motif is specific for class IIa bacteriocins. A BLAST search revealed that this bacteriocin was similar to leucocin C from Leuconostoc mesenteroides. Leucocin C-specific primers were designed and a single PCR product was amplified. Analysis of the nucleotide sequence has revealed a putative peptide differing by only one amino acid residue from the sequence of leucocin C. No identical peptide or protein has been reported in the literature, and this peptide, termed leucocin C-607, was therefore considered to be a new variant of leucocin C produced by Leuc. pseudomesenteroides 607. Another antibacterial peptide purified from the same culture supernatant had a molecular size of 3007.7 or 3121.97 Da. However, detailed information regarding this second peptide remains to be determined. Distinct characteristics, such as heat stability and inhibitory spectrum, were observed for the two bacteriocins produced by Leuc. pseudomesenteroides 607. These results suggested that Leuc. pseudomesenteroides 607 produces leucocin C-607 along with another unknown bacteriocin.