Identification of Functional Single Nucleotide Polymorphisms in Porcine HSD17B14 Gene Associated with Estrus Behavior Difference between Large White and Mi Gilts.
Identification of Functional Single Nucleotide Polymorphisms in Porcine HSD17B14 Gene Associated with Estrus Behavior Difference between Large White and Mi Gilts.
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与大白猪和米后备母猪发情行为差异相关的猪 HSD17B14 基因功能性单核苷酸多态性的鉴定
DOI：
10.3390/biom10111545
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发表时间：
2020-11-12
期刊：
影响因子：
5.5
通讯作者：
Zhou B
中科院分区：
文献类型：
--
作者：
Gao S;Tao R;Tong X;Xu Q;Zhao J;Guo Y;Schinckel AP;Zhou B
类固醇激素水平与发情行为相关，这会影响猪的适时配种和繁殖效率。17β - 羟基类固醇脱氢酶14型（HSD17B14）调节类固醇的合成与代谢。为鉴定猪HSD17B14基因中的功能性单核苷酸多态性（SNP），采集了大白猪和梅山母猪的耳部组织以提取基因组DNA。利用不同长度的HSD17B14基因截短启动子，通过双荧光素酶报告系统测定启动子活性。将载体HSD17B14Phe或HSD17B14Val转染到猪颗粒细胞（GCs）中。确定核心启动子区域在−72 bp至−218 bp之间。7个SNP中有6个在大白猪和梅山母猪间的等位基因频率存在显著差异。与具有突变基因型GG的质粒相比，具有rs329427898野生基因型AA的质粒维持的启动子活性比例更低，而具有rs319864566野生基因型TT的质粒比具有突变基因型CC的质粒具有更高的启动子活性。一个错义突变（Phe73Val）导致HSD17B14蛋白的结构动力学和功能发生变化。高表达的HSD17B14Val将较少的雌二醇降解为雌酮，而相对低表达的HSD17B14Phe将较多的雌二醇降解为雌酮，这表明HSD17B14Phe的蛋白活性高于HSD17B14Val。此外，HSD17B14Phe组猪颗粒细胞的凋亡率更高。HSD17B14基因可作为猪发情行为的候选分子标记。
Steroid hormone levels are associated with estrous behavior, which affects timely mating and reproductive efficiency in pigs. 17β-hydroxysteroid dehydrogenase type 14 (HSD17B14) modulates steroid synthesis and metabolism. To identify the functional single nucleotide polymorphisms (SNPs) in the porcine HSD17B14 gene, ear tissues from Large White and Mi gilts were collected to extract genomic DNA. Variable lengths of truncated promoter of HSD17B14 gene were used to determine the promoter activity by a dual luciferase reporter system. The vector HSD17B14Phe or HSD17B14Val was transfected into porcine granulosa cells (GCs). The core promoter region was identified between −72 bp and −218 bp. Six of seven SNPs had significant differences of allele frequency between Large White and Mi gilts. The plasmids with the wild genotype AA of rs329427898 maintained a smaller fraction of promoter activity compared with the plasmids with the mutant genotype GG, while the plasmids with wild the genotype TT of rs319864566 had a greater promoter activity than the plasmids with the mutant genotype CC. A missense mutation (Phe73Val) caused changes in the structural dynamics and function of the HSD17B14 protein. The highly expressed HSD17B14Val degraded less estradiol into estrone, while the relatively lowly expressed HSD17B14Phe degraded more estradiol into estrone, suggesting the protein activity of HSD17B14Phe was greater than that of HSD17B14Val. Moreover, the HSD17B14Phe group has a greater apoptosis rate of porcine GCs. The HSD17B14 gene could been used as a candidate molecular marker for estrus behavior in pigs.
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影响因子：
6.6
作者：
Juneja, Manisha;Ilm, Katharina;Stein, Ulrike
通讯作者：
Stein, Ulrike
影响因子：
2.9
作者：
Bongiorni S;Tilesi F;Bicorgna S;Iacoponi F;Willems D;Gargani M;D'Andrea M;Pilla F;Valentini A
通讯作者：
Valentini A
影响因子：
3.3
作者：
Knauer, M. T.;Cassady, J. P.;See, M. T.
通讯作者：
See, M. T.
中国米猪与欧洲长白猪发情征象及雌激素代谢相关基因的遗传差异
影响因子：
1.7
作者：
Chu, Q.;Liang, T.;Zhou, B.
通讯作者：
Zhou, B.
影响因子：
--
作者：
Ciesiółka S;Budna J;Jopek K;Bryja A;Kranc W;Borys S;Jeseta M;Chachuła A;Ziółkowska A;Antosik P;Bukowska D;Brüssow KP;Bruska M;Nowicki M;Zabel M;Kempisty B
通讯作者：
Kempisty B