The molecular cloning of a type II regulatory subunit of the cAMP-dependent protein kinase from rat skeletal muscle and mouse brain.
The molecular cloning of a type II regulatory subunit of the cAMP-dependent protein kinase from rat skeletal muscle and mouse brain.
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DOI：
10.1073/pnas.84.15.5192
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发表时间：
1987-08
影响因子：
11.1
通讯作者：
J. Scott;Moira B. Glaccum;M. Zoller;Michael D. UHLERt;D. Helfman;G. S. MCKNIGHTt;E. Krebs
中科院分区：
文献类型：
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作者：
J. Scott;Moira B. Glaccum;M. Zoller;Michael D. UHLERt;D. Helfman;G. S. MCKNIGHTt;E. Krebs
利用特异性47碱基寡核苷酸探针从大鼠骨骼肌文库中分离到camp依赖性蛋白激酶（ATP:protein phosphotransferase, EC 2.7.1.37） II型调控亚基的cDNA克隆。大鼠cDNA全长1.2千碱基（kb），包含1.113 kb的开放阅读框，占该分子编码区域的92%。然后用nick翻译的大鼠cDNA从包含1.143 kb开放阅读框的脑文库中分离出小鼠RII cDNA克隆。由于这两种cdna都缺乏完整的编码序列，因此RII编码区的其余部分是从一个15kb的小鼠基因组克隆中获得的。小鼠RII编码区包含1.2 kb，对应一个400个氨基酸的51.141 kDa蛋白。小鼠cDNA杂交成两种mRNA，一种是仅在睾丸中观察到的2.4 kb形式，另一种是在包括睾丸在内的广泛组织中发现的6.0 kb形式。
A cDNA clone for a type II regulatory (R) subunit of the cAMP-dependent protein kinase (ATP:protein phosphotransferase, EC 2.7.1.37) was isolated from a rat skeletal muscle library using a specific 47-base oligonucleotide probe. The rat cDNA was 1.2 kilobases (kb) in length and contained an open reading frame of 1.113 kb representing 92% of the coding region of the molecule. Nick-translated rat cDNA was then used to isolate a mouse RII cDNA clone from a brain library that contained an open reading frame of 1.143 kb. Because both cDNAs lacked complete coding sequences, the remainder of the RII coding region was obtained from a 15-kb mouse genomic clone. The mouse RII coding region contains 1.2 kb corresponding to a 400-amino acid protein of 51.141 kDa. The mouse cDNA hybridizes to two mRNA species, a 2.4-kb form that was only observed in testis and a 6.0-kb form found in a wide range of tissues, including testis.