Ultrastructure of orexin-1 receptor immunoreactivities in the spinal cord dorsal horn
Ultrastructure of orexin-1 receptor immunoreactivities in the spinal cord dorsal horn
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DOI：
10.1016/j.peptides.2004.05.018
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发表时间：
2004-08-01
期刊：
影响因子：
3
通讯作者：
Shioda, S
中科院分区：
文献类型：
--
作者：
Guan, JL;Wang, QP;Shioda, S
应用光镜和电镜技术观察了大鼠脊髓背角食欲素1受体样免疫反应（OX 1 R-LI）神经元的超微结构。在光镜水平，最重的免疫染色的OX 1 R-LI神经元被发现在脊髓的前角，而一些免疫染色的轮廓，包括神经纤维和小神经元，也被发现在背角。在电子显微镜水平，OX 1 R-LI胞体被确定含有大量的致密核心囊泡，比任何其他细胞器更重的免疫染色。在OX 1 R-LI神经元的轴突终末内也发现了类似的囊泡。部分OX 1 R-LI神经元的胞体和树突接受免疫阴性轴突终末的突触。发现这些突触大多形状不对称。偶见一些OX 1 R-LI轴突终末在树突上形成突触，这些树突在某些情况下为OX 1 R-LI，而在另一些情况下为免疫阴性。OX 1 R-LI轴突终末所形成的突触在形态上既有对称性，也有不对称性。这些结果提供了坚实的形态学证据，表明OX 1 R在致密核心囊泡中从胞体转运到轴突终末，并且脊髓背角中的OX 1 R-LI神经元与其他OX 1 R-LI神经元以及其他神经元类型具有复杂的突触关系。(C)2004年爱思唯尔公司All rights reserved.
The ultrastructural properties of orexin 1-receptor-like immunoreactive (OX1R-LI) neurons in the dorsal horn of the rat spinal cord were examined using light and electron microscopy techniques. At the light microscopy level, the most heavily immunostained OX1R-LI neurons were found in the ventral horn of the spinal cord, while some immunostained profiles, including nerve fibers and small neurons, were also found in the dorsal horn. At the electron microscopy level, OX1R-LI perikarya were identified containing numerous dense-cored vesicles which were more heavily immunostained than any other organelles. Similar vesicles were also found within the axon terminals of the OX1R-LI neurons. The perikarya and dendrites of some of the OX1R-LI neurons could be seen receiving synapses from immunonegative axon terminals. These synapses were found mostly asymmetric in shape. Occasionally, some OX1R-LI axon terminals were found making synapses on dendrites that were OX1R-LI in some cases and immunonegative in others. The synapses made by OX1R-LI axon terminals were found both asymmetric and symmetric in appearance. The results provide solid morphological evidence that OX1R is transported in the dense-cored vesicles from the perikarya to axon terminals and that OX1R-LI neurons in the dorsal horn of the spinal cord have complex synaptic relationships both with other OX1R-LI neurons as well as other neuron types. (C) 2004 Elsevier Inc. All rights reserved.