AHMT functionalized g-C3N4 quantum dots/Au based localized surface plasmon resonance sensors for ultrasensitive and rapid detection of adrenaline
AHMT functionalized g-C3N4 quantum dots/Au based localized surface plasmon resonance sensors for ultrasensitive and rapid detection of adrenaline
复制标题
AHMT 功能化 g-C3N4 量子点/Au 基局部表面等离子体共振传感器，用于超灵敏快速检测肾上腺素
DOI：
10.1016/j.snb.2023.133906
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Doong Ruey-an
中科院分区：
文献类型：
--
作者：
Dega Naresh Kumar;Ganganboina Akhilesh Babu;Venkatesan Prashanth;Roy Deblina;Tran Hai Linh;Doong Ruey-an
肾上腺素(ADR)是导致应激综合征的一种神经递质/激素，血液中的浓度水平是健康后果的指标。因此，基于4-氨基-3-肼-5-巯基-1，2，4-三氮唑(Ahmt)存在下，高荧光g-C3N4量子点(CNQD)与表面等离子体共振金纳米颗粒(AuNPs)之间的相互作用，开发了一种简单、经济的纳米传感器，用于ADR的超灵敏和选择性检测。将Ahmt功能化到CNQD和AuNPs上后，Ahmt可以作为识别分子与ADR通过氢键连接，导致CNQD到AuNP的非辐射能量转移，从而猝灭荧光强度。猝灭效率高度依赖于ADR浓度和AuNPs的大小。当45 nm的AuNPs与CNQD结合时，ADR的检测获得了良好的线性关系，动态范围宽(10 PM-100µM)，检测下限为7 PM。此外，所研制的传感器对25种潜在的干扰具有很高的选择性。这些结果证明了AHMT-CNQD-AuNP传感探针用于ADR检测的优越性，可用于设计一种新开发的用于生物医学应用中ADR相关疾病的早期检测和治疗诊断的纳米传感平台。
Adrenaline (ADR) is a neurotransmitter/hormone responsible for stress syndrome and the concentration level in blood is an indication of health consequences. Herein, a simple and cost-effective nanosensors based on the interaction between highly fluorescent g-C3N4quantum dots (CNQDs) and surface plasmon resonance gold nanoparticles (AuNPs) in the presence of 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole (AHMT) was developed for ultra-sensitive and selective detection of ADR. After functionalization of AHMT onto both CNQDs and AuNPs, AHMT can serve as the recognizer to link with ADR by hydrogen bond, resulting in the non-radiative energy transfer from CNQD to AuNP to quench the fluorescence intensity. The quenching efficiency is highly dependent on ADR concentration and the size of AuNPs. When 45 nm AuNPs is combined with CNQDs, an excellent linear relationship with a wide dynamic range of (10 pM – 100 µM) and a low limit of detection of 7 pM is obtained for ADR detection. Moreover, the developed sensor exhibits high selectivity towards ADR detection over 25 potential interferences. These findings have demonstrated the superiority of AHMT-conjugated CNQD-AuNP sensing probe for ADR detection, which can be applied to design of a newly developed nanosensing platform for the early detection and therapeutic diagnosis of ADR associated diseases in biomedical applications.