ALKALINE-O-]N-TRANSACYLATION - A NEW METHOD FOR THE QUANTITATIVE DEACYLATION OF PHOSPHOLIPIDS
ALKALINE-O-]N-TRANSACYLATION - A NEW METHOD FOR THE QUANTITATIVE DEACYLATION OF PHOSPHOLIPIDS
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DOI：
10.1042/bj1950301
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发表时间：
1981-01-01
影响因子：
4.1
通讯作者：
DAWSON, RMC
中科院分区：
文献类型：
--
作者：
CLARKE, NG;DAWSON, RMC
通过与含有一甲胺、甲醇和水的试剂孵育，实现了磷脂的定量O-脱酰化。该反应主要是O → O反应。形成N-甲基脂肪酸酰胺的N-转酰作用。该试剂可以通过挥发容易地除去，并且在限定的条件下，不发生含磷脱酰产物的二次分解。通过哺乳动物组织O-二酰化磷脂的脱酰化得到的水溶性磷化合物，通过使用挥发性pH 9缓冲液的一维纸离子电泳完全分离。用薄层色谱法对Hg ~（2+）催化水解前后的O-脱酰烷基和烯基磷脂进行了检测。通过这些方法对大鼠脑磷脂进行的完整分析与通过其他程序获得的结果密切一致。
Quantitative O-deacylation of phospholipids has been achieved by incubation with a reagent containing monomethylamine, methanol and water. The reaction is primarily an O .fwdarw. N-transacylation with N-methyl fatty acid amides being formed. The reagent can be removed easily by volatilization, and under defined conditions no secondary decomposition of the phorphorus-containing deacylation products occurs. The water-soluble phosphorus compounds, derived by deacylation of mammalian tissue O-diacylated phospholipids, were completely separated by a single-dimensional paper ionophoresis with a volatile pH 9 buffer. The O-deacylated alkyl and alkenyl phospholipids were examined by TLC before and after catalytic hydrolysis with Hg2+. A complete analysis of rat brain phospholipids by these methods agrees closely with that obtained by other procedures.