The effect of superoxide anion and hydrogen peroxide imbalance on prostate cancer: an integrative in vivo and in vitro analysis.
The effect of superoxide anion and hydrogen peroxide imbalance on prostate cancer: an integrative in vivo and in vitro analysis.
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DOI：
10.1007/s12032-015-0700-1
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发表时间：
2015-11-01
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影响因子：
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通讯作者：
da Cruz, Ivana Beatrice Manica
中科院分区：
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作者：
Berto, Maiquidieli Dal;Bica, Claudia Giuliano;da Cruz, Ivana Beatrice Manica
SOD2失衡对与遗传变异相关的前列腺癌风险的流行病学影响已有研究。然而，我们没有发现先前的研究阐明SOD2对前列腺癌作用的性质。在这里，我们进行了体内和体外综合方案，分析了诊断时Ala16Val-SOD2多态性与前列腺癌侵袭性之间的关系，并使用百草枯和卟啉处理的DU-145 PC细胞株评估了这种不平衡对PC增殖的影响。在药理学模型中，使用百草枯增加超氧阴离子水平，卟啉是SOD2类似物。结果证实了超氧化物-过氧化氢失衡对PC细胞生物学的影响，因为卟啉降低了细胞增殖，两种处理都调节了抗氧化基因。因此，我们的结果证实了先前的建议，即氧化还原状态的改变可以用于治疗PC。
The epidemiological impact of SOD2 imbalance on prostate cancer (PC) risk associated with genetic variations has previously been studied. However, we found no previous studies clarifying the nature of SOD2 effects on prostate cancer. Here, we performed integrated in vivo and in vitro protocols that analyzed the association between Ala16Val-SOD2 polymorphism and prostate cancer aggressiveness at the time of diagnosis and evaluated the effect of the imbalance on PC proliferation using the DU-145 PC cell line treated with paraquat and porphyrin. In the pharmacological model, paraquat was used to increase superoxide anion levels and porphyrin was the SOD2 analog. The results confirmed the impact of superoxide-hydrogen peroxide imbalance on PC cell biology since porphyrin decreased cell proliferation and both treatments modulated antioxidant genes. Therefore, our results corroborate previous suggestions that alteration of redox status could be exploited therapeutically in the treatment of PC.