MOLECULAR-CLONING, NUCLEOTIDE-SEQUENCE, AND EXPRESSION OF THE GENE ENCODING HUMAN EOSINOPHIL DIFFERENTIATION FACTOR (INTERLEUKIN-5)
MOLECULAR-CLONING, NUCLEOTIDE-SEQUENCE, AND EXPRESSION OF THE GENE ENCODING HUMAN EOSINOPHIL DIFFERENTIATION FACTOR (INTERLEUKIN-5)
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DOI：
10.1073/pnas.84.19.6629
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发表时间：
1987-10-01
影响因子：
11.1
通讯作者：
YOUNG, IG
中科院分区：
文献类型：
--
作者：
CAMPBELL, HD;TUCKER, WQJ;YOUNG, IG
从人嗜酸性粒细胞分化因子（EDF）基因组文库中克隆到人嗜酸性粒细胞分化因子（EDF）基因。利用小鼠EDF cDNA克隆作为探针检测噬菌体EMBL3A。测定了一个3.2千碱基BamHI片段的DNA序列。该基因含有三个内含子。预测的134个氨基酸序列与最近报道的人类白细胞介素5相同，但与其他已知的造血生长调节剂没有显著的同源性。氨基酸序列具有强同源性（.simeq）。70%的同源性)与小鼠EDF的同源性。重组人EDF基因转染猴COS细胞后表达，刺激了正常人骨髓中嗜酸性粒细胞和嗜酸性粒细胞集落的产生，但对中性粒细胞和单核细胞（单核细胞和淋巴样细胞）的产生没有影响。在髓系造血中，人EDF对嗜酸性粒细胞谱系的明显特异性与人白细胞介素3、粒细胞/巨噬细胞和粒细胞集落刺激因子的特性形成对比，但与小鼠EDF的生物学特性直接相似。因此，人类EDF代表了一种独特的造血生长因子，可以在嗜酸性粒细胞的调节中发挥核心作用。
The human eosinophil differentiation factor (EDF) gene was cloned from a genomic library in .lambda. phage EMBL3A by using a murine EDF cDNA clone as a probe. The DNA sequence of a 3.2-kilobase BamHI fragment spanning the gene was determined. The gene contains three introns. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin 5 but shows no significant homology with other known hemopoietic growth regulators. The amino acid sequence shows strong homology (.simeq. 70% identity) with that of murine EDF. Recombinant human EDF, expressed from the human EDF gene after transfection into monkey COS cells, stimulated the production of eosinophils and eosinophil colonies from normal human bone marrow but had no effect on the production of neutrophils or mononuclear cells (monocytes and lymphoid cells). The apparent specificity of human EDF for the eosinophil lineage in myeloid hemopoiesis contrasts with the properties of human interleukin 3 and granulocyte/macrophage and granulocyte colony-stimulating factors but is directly analogous to the biological properties of murine EDF. Human EDF therefore represents a distinct hemopoietic growth factor that could play a central role in the regulation of eosinophila.