Patients with papular urticaria have IgG antibodies to bedbug (Cimex lectularius) antigens
Patients with papular urticaria have IgG antibodies to bedbug (Cimex lectularius) antigens
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DOI：
10.1007/s00436-005-0076-9
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发表时间：
2006-05-01
影响因子：
2
通讯作者：
Ali, NMS
中科院分区：
文献类型：
--
作者：
Abdel-Naser, M;Lotfy, RA;Ali, NMS
采用免疫印迹法（Immunoblotting），将臭虫（Cimex lectularius）抗唾液腺蛋白IgG和IgE与丘疹性荨麻疹患者（I组）、无丘疹性荨麻疹的兄弟姐妹（IIa组）、患者父母（IIb组）和健康对照（III组）血清中的蚊子（库蚊）和跳蚤（激蚤）抗原进行比较。Anti-C。1组40%的小鼠在66和10 kDa的水平上被显著识别，其余的在45 - 107 kDa之间。IIa组明显反应70 kDa（57.1%）。IIb组以21和8.5 kDa反应（26.7%）。60%的IIb组和100%的III组显著识别12.5 kDa波段。ⅰ组、ⅱ组（115,7 kDa）和ⅲ组（58,50 kDa）在18-105 kDa范围内显著识别出抗库蚊IgG。Anti-P。iritans IgG被I组（100、70 kDa）和IIa组（60、35 kDa）显著识别。ⅰ组和ⅲ组的IgE反应分别局限于115和54 kDa，ⅱ组的IgE反应分别为68和58 kDa。结论在丘疹性荨麻疹中，IgG抗体对白化棘球蚴、淡红色棘球蚴和刺激性棘球蚴均存在，可能参与其发病机制。
IgG and IgE against salivary gland proteins of bedbug (Cimex lectularius) were assessed in comparison with mosquito (Culex pipiens) and flea (Pulex irritans) antigens in the sera of papular urticaria patients (group I), siblings without papular urticaria (group IIa), patients' parents (group IIb), and healthy controls (group III) (Immunoblotting). Anti-C. lectularius IgG was significantly recognized at 66 and 10 kDa in 40% of group I, besides others ranging from 45 to 107 kDa. Group IIa significantly reacted with 70 kDa (57.1%). Group IIb reacted with 21 and 8.5 kDa (26.7%). Sixty percent of group IIb and 100% of group III significantly identified a band of 12.5 kDa. IgG against C. pipiens was significantly recognized at a range of 18-105 kDa in group I, IIb (115, 7 kDa), and III (58, 50 kDa). Anti-P. irritans IgG was significantly recognized by group I (100, 70 kDa) and group IIa (60, 35 kDa). IgE response was confined to C. pipiens at 115 and 54 kDa in groups I and III, respectively, besides 68 and 58 kDa in group IIa. It is concluded that IgG is present against C. lectularius, C. pipiens, and P. irritans in papular urticaria and may contribute to its pathogenesis.