Association Analysis of Myosin Heavy-chain Genes mRNA Transcription with the Corresponding Proteins Expression of Longissimus Muscle in Growing Pigs
Association Analysis of Myosin Heavy-chain Genes mRNA Transcription with the Corresponding Proteins Expression of Longissimus Muscle in Growing Pigs
复制标题
生长猪肌球蛋白重链基因mRNA转录与最长肌相应蛋白表达的关联分析
DOI：
10.5713/ajas.15.0259
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发表时间：
2016-04-01
期刊：
影响因子：
--
通讯作者：
Xu, Z. W.
中科院分区：
文献类型：
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作者：
Men, X. M.;Deng, B.;Xu, Z. W.
本研究旨在探讨猪出生后生长过程中背最长肌（LM）中MyHC mRNA转录及其相应蛋白表达的相关性。选用5头杜洛克×大约克夏（DLY）杂交猪，分别于生后7、30、60、120和180日龄屠宰取样。通过间接酶联免疫吸附试验（ELISA）定量检测肌肉中MyHCs蛋白含量，通过实时聚合酶链反应（PCR）定量检测肌球蛋白重链（MyHCs）mRNA丰度。我们分别计算了两个水平下每种MyHC占四种MyHC总数的比例（%）。此外，还测定了几种关键能量代谢酶的活性。结果表明，随着猪出生后年龄的增长，特别是出生后早期，LM中MyHC I、IIa、IIx和IIb的mRNA转录和蛋白表达均呈现出明显的变化，且其mRNA转录比蛋白表达更易受影响。各MyHC mRNA的相对比例与其相应蛋白的相对比例呈显著正相关（p
The goal of this work was to investigate the correlations between MyHC mRNA transcription and their corresponding protein expressions in porcine longissimus muscle (LM) during postnatal growth of pigs. Five DLY (DurocxLandracexYorkshire) crossbred pigs were selected, slaughtered and sampled at postnatal 7, 30, 60, 120, and 180 days, respectively. Each muscle was subjected to quantity MyHCs protein contents through an indirect enzyme-linked immunosorbent assay (ELISA), to quantity myosin heavy-chains (MyHCs) mRNA abundances using real-time polymerase chain reaction. We calculated the proportion (%) of each MyHC to total of four MyHC for two levels, respectively. Moreover, the activities of several key energy metabolism enzymes were determined in LM. The result showed that mRNA transcription and protein expression of MyHC I, IIa, IIx and IIb in LM all presented some obvious changes with postnatal aging of pigs, especially at the early stage after birth, and their mRNA transcriptions were easy to be influenced than their protein expressions. The relative proportion of each MyHC mRNA was significantly positively related to that of its corresponding protein (p