Effects of myofiber isolation technique on sarcolemma biomechanics.
Effects of myofiber isolation technique on sarcolemma biomechanics.
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DOI：
10.2144/btn-2020-0087
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发表时间：
2020-11
期刊：
影响因子：
2.7
通讯作者：
Lovering RM
中科院分区：
文献类型：
--
作者：
Garcia-Pelagio KP;Pratt SJP;Lovering RM
分离的肌纤维通常用于了解体内骨骼肌的功能。这可以涉及从解剖或酶促解离获得的单个分离的肌纤维。通过解剖进行分离可以控制肌节长度并保留肌腱附着，但劳动强度大、耗时且产生的存活肌纤维很少。相比之下，酶促解离快速而容易，产生数百个肌纤维，更重要的是减少了研究所需的肌肉/动物数量。肌膜的生物力学特性已被研究使用肌纤维从伸趾长肌，但这已被限制到解剖肌纤维，使数据收集缓慢和困难。我们已经修改了这个工具来进行生物力学测量的肌膜分离的肌纤维从屈趾短肌。弹性测量技术的改进使我们能够以更高的通量获得酶促解离肌纤维中肌膜的生物力学测量结果。
Isolated myofibers are commonly used to understand the function of skeletal muscle in vivo. This can involve single isolated myofibers obtained from dissection or from enzymatic dissociation. Isolation via dissection allows control of sarcomere length and preserves tendon attachment but is labor-intensive, time-consuming and yields few viable myofibers. In contrast, enzymatic dissociation is fast and facile, produces hundreds of myofibers, and more importantly reduces the number of muscles/animals needed for studies. Biomechanical properties of the sarcolemma have been studied using myofibers from the extensor digitorum longus, but this has been limited to dissected myofibers, making data collection slow and difficult. We have modified this tool to perform biomechanical measurements of the sarcolemma in dissociated myofibers from the flexor digitorum brevis. Modifications of the elastimetry technique allowed us to obtain biomechanical measurements of the sarcolemma in enzymatically dissociated myofibers at a much higher throughput.