MULTIFREQUENCY EPR EVIDENCE FOR A BINUCLEAR CU-A CENTER IN CYTOCHROME-C-OXIDASE - STUDIES WITH A CU-63-ENRICHED AND CU-65-ENRICHED, SOLUBLE DOMAIN OF THE CYTOCHROME-BA(3) SUBUNIT-II FROM THERMUS-THERMOPHILUS
MULTIFREQUENCY EPR EVIDENCE FOR A BINUCLEAR CU-A CENTER IN CYTOCHROME-C-OXIDASE - STUDIES WITH A CU-63-ENRICHED AND CU-65-ENRICHED, SOLUBLE DOMAIN OF THE CYTOCHROME-BA(3) SUBUNIT-II FROM THERMUS-THERMOPHILUS
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DOI：
10.1006/bbrc.1995.1938
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发表时间：
1995-07-06
影响因子：
3.1
通讯作者：
VANNGARD, T
中科院分区：
文献类型：
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作者：
FEE, JA;SANDERS, D;VANNGARD, T
我们记录了Cu-63和Cu-65标记的T.嗜热菌在最高频率（34.03 GHz）下获得的光谱显示没有超精细结构，并且名义上是轴向的，表观g（z）类似于2.18，g（xy）类似于2.00。在最低频率（3.93 GHz）处获得的光谱显示出丰富的超精细结构。光谱分析表明，所观察到的分裂来自于未成对电子与两个Cu核的相互作用，并支持Cu-A是混合价[Cu（II）/Cu（I）]络合物的概念，其中未成对电子自旋均匀分布在两个Cu离子上。(C)出版社：Academic Press
We have recorded multi-frequency EPR spectra of Cu-63- and Cu-65-labeled, water-soluble Cu-A-protein from the cytochrome ba(3) of T. thermophilus. The spectrum taken at the highest frequency (34.03 GHz) shows no hyperfine structure and is nominally axial with apparent g(z) similar to 2.18 and g(xy) similar to 2.00. The spectrum taken at the lowest frequency (3.93 GHz) shows a rich hyperfine structure. Analyses of the spectra show that the observed splitting arises from an interaction of the unpaired electron with two Cu nuclei and support the notion that Cu-A is a mixed-valent [Cu(II)/Cu(I)] complex in which the unpaired electronic spin is distributed evenly over the two Cu ions. (C) 1995 Academic Press, Inc.