A Rice NBS-ARC Gene Conferring Quantitative Resistance to Bacterial Blight Is Regulated by a Pathogen Effector-Inducible miRNA
A Rice NBS-ARC Gene Conferring Quantitative Resistance to Bacterial Blight Is Regulated by a Pathogen Effector-Inducible miRNA
复制标题
水稻 NBS-ARC 基因赋予对细菌性枯萎病的定量抗性，并受病原体效应子诱导的 miRNA 调节
DOI：
10.1016/j.molp.2020.09.015
复制
发表时间：
2020-12-07
期刊：
影响因子：
27.5
通讯作者：
Zhai, Wenxue
中科院分区：
文献类型：
--
作者：
Jiang, Guanghuai;Liu, Dongfeng;Zhai, Wenxue
米黄单胞菌(Xanthomonas Oryzae)Otyzae(Xoo)在世界范围内引起水稻枯萎病，造成严重的作物损失。然而，目前还没有克隆到与白粉病抗性相关的数量性状基因。在这里，我们报道了基于图谱的一个QTL的克隆，其中NBS8R基因赋予了对Xoo的数量抗性。NBS8R编码一个NB-ARC蛋白，参与病原体/微生物相关的分子模式触发免疫，其表达受非TAL效应子XopQ诱导的OSA-miR1876通过DNA甲基化调节。野生稻和栽培稻的NBS8R序列分析表明，NBS8R的5‘非编码区中的Osa-miR1876结合位点是偶然插入的，并且在整个进化过程中与Osa-miR1876发生了变异。NBS8R和XopQ诱导的Osa-miR1876之间的相互作用部分符合Z字形模型，揭示了数量基因也可能遵循这一模型来控制先天免疫反应或基础抗病，这可能在利用现有的含有NBS8R基因但没有Osa-miR1876结合位点的地方品种进行白叶枯病抗性育种中具有价值。
The bacterium Xanthomonas oryzae pv. Otyzae (Xoo) causes blight in rice worldwide, resulting in significant crop loss. However, no gene underlying a quantitative trait locus (QTL) for resistance against Xoo has been cloned yet. Here, we report the map-based cloning of a QTL, in which the NBS8R gene confers quantitative resistance to Xoo. NBS8R encodes an NB-ARC protein, which is involved in pathogen/microbe-associated molecular pattern-triggered immunity and whose expression is regulated by non-TAL effector XopQ-inducible Osa-miR1876 through DNA methylation. Sequence analysis of NBS8R in wild rice species and rice cultivars suggests that the Osa-miR1876 binding sites in the 5' UTR of NBS8R are inserted by chance and have undergone variations with Osa-miR1876 throughout evolution. The interaction between NBS8R and XopQ-inducible Osa-miR1876 is partially in keeping with the zigzag model, revealing that quantitative genes may also follow this model to control the innate immune response or basal disease resistance, and may prove valuable in utilizing the existing landraces that harbor the NBS8R gene but with no Osa-miR1876 binding site in rice breeding for bacterial blight resistance.