Highly persistent label-retaining cells in the hair follicles of mice and their fate following induction of anagen
Highly persistent label-retaining cells in the hair follicles of mice and their fate following induction of anagen
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DOI：
10.1046/j.1523-1747.1999.00537.x
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发表时间：
1999-04-01
影响因子：
6.5
通讯作者：
Potten, CS
中科院分区：
文献类型：
--
作者：
Morris, RJ;Potten, CS
我们在小鼠毛囊中发现了一些异常持久的标记保留细胞，并研究了它们在毛发生长中的作用。新生小鼠表皮和毛囊完全标记后14个月连续切片的背侧绒毛毛囊三维重建揭示了与第一代毛囊相关的高度持久的标记保留细胞的存在，第一代毛囊参与第一波毛发的产生，通常称为隆突。标记保留细胞最常见于第一代毛囊的腹面，距基部5个细胞位置，靠近立毛肌的附着部位。在毛管、皮脂腺和毛胚中未发现标记保留细胞。这些标记保留细胞保留在毛囊诱导生长期后拔毛。令人惊讶的是，它们不是采摘后第一波有丝分裂活动的一部分，而是在采摘后42小时开始进行有丝分裂。通过拔毛诱导生长期后48 h，在毛胚中未发现标记保留细胞或其标记的子代细胞，但即使在21 d后完成毛发生长周期后，标记保留细胞仍保留在其皮脂腺下毛囊位置。因此，这些标记保留细胞不太可能促进新生长期卵泡的形成。
We have identified some unusually persistent label-retaining cells in the hair follicles of mice, and have investigated their role in hair growth. Three-dimensional reconstruction of dorsal underfur follicles from serial sections made 14 mo after complete labeling of epidermis and hair follicles in neonatal mice disclosed the presence of highly persistent label-retaining cells associated with the first-generation follicle involved in the production of the first wave of hairs, commonly called the bulge. The label-retaining cells were most often found on the ventral surface of the first-generation follicle, five cell positions from the base, near the attachment site of the arrector pilorum muscle. No label-retaining cells were found in the hair canal, sebaceous gland, or hair germ. These label-retaining cells remained in the follicle following induction of anagen by plucking of the hairs. Surprisingly, they were not part of the first wave of mitotic activity following plucking, but instead underwent mitosis beginning 42 h after plucking. Label-retaining cells or their labeled daughters were not found in the hair germs through 48 h following induction of anagen by plucking, but instead remained in their subsebaceous follicular location even upon completion of the hair growth cycle 21 d later. These label-retaining cells are, therefore, unlikely to contribute to the-formation of a new anagen follicle.