Modulation of aspect ratio for complete separation in an inertial microfluidic channel.
Modulation of aspect ratio for complete separation in an inertial microfluidic channel.
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由于惯性微流体在细胞分离和分选方面的巨大应用前景，近年来引起了人们的极大兴趣。尽管引起了强烈的关注，但所报道的设备的效率适中和纯度低阻碍了它们的广泛接受。在这项工作中，我们报道了一个简单的惯性微流控系统，具有高效率（>99%）和纯度（>90%）。我们的系统建立在两阶段惯性迁移的概念上，它允许在微通道内精确预测粒子或细胞的位置。我们的设计通过调制通道宽高比来操纵惯性平衡位置，以实现完全的分离。在这里，我们成功地证明了颗粒的完全分离，并分离了血液中含有人类前列腺上皮肿瘤（HPET）细胞的罕见细胞。基于平面结构，大的分离间距和可预测的聚焦，我们设想了有前途的应用，并且我们的系统易于与现有的芯片实验室系统集成，用于细胞分离。
Inertial microfluidics has been attracting considerable interest in recent years due to immensely promising applications in cell separations and sorting. Despite the intense attention, the moderate efficiencies and low purity of the reported devices have hindered their widespread acceptance. In this work, we report on a simple inertial microfluidic system with high efficiency (>99%) and purity (>90%). Our system builds on the concept of two-stage inertial migration which permits precise prediction of particle or cell position within the microchannel. Our design manipulates the inertial equilibrium positions by modulating channel aspect ratio to achieve a complete separation. Here, we successfully demonstrate a complete separation of particles and isolation of rare cells in blood spiked with human prostate epithelial tumor (HPET) cells. Based on the planar structure, large separation spacing and predictable focusing, we envision promising applications and easy integration of our system with existing lab-on-a-chip systems for cell separations.