Use of polymerase chain reaction in the diagnosis of tuberculosis in cats and dogs
Use of polymerase chain reaction in the diagnosis of tuberculosis in cats and dogs
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DOI：
10.1136/vr.138.12.276
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发表时间：
1996-03-23
影响因子：
2.2
通讯作者：
Dominguez, L
中科院分区：
文献类型：
--
作者：
Aranaz, A;Liebana, E;Dominguez, L
对怀疑患有肺结核的4只狗和4只猫的样本进行了组织病理学、细菌培养和聚合酶链反应（FCR）处理。从组织样本中提取DNA的一种简单、快速的方法用于两种旨在确认结核病诊断的PCR检测。聚合酶链式反应（PCR）检测出所有培养阳性标本，无假阳性结果，PCR技术成功地直接检测出结核分枝杆菌复合体，并将诊断所需时间缩短至2天。
Samples from four dogs and four cats suspected of having tuberculosis were processed for histopathology, bacterial culture and polymerase chain reaction (FCR). A simple, rapid method for the extraction of DNA from tissue samples was used in two PCR assays designed to confirm the diagnosis of tuberculosis. The PCR assays detected all the culture-positive samples from these animals and no false positive results were obtained, The PCR technique was successful for the direct detection of organisms from the Mycobacterium tuberculosis complex and reduced the time needed for a diagnosis to two days.