Molecular markers for tolerance of European ash (Fraxinus excelsior) to dieback disease identified using Associative Transcriptomics.
Molecular markers for tolerance of European ash (Fraxinus excelsior) to dieback disease identified using Associative Transcriptomics.
复制标题
使用联想转录组学鉴定的欧洲灰分（fraxinus excelsior）耐受性疾病的耐受性的分子标记。
DOI：
10.1038/srep19335
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发表时间：
2016-01-13
影响因子：
4.6
通讯作者：
Bancroft I
中科院分区：
文献类型：
--
作者：
Harper AL;McKinney LV;Nielsen LR;Havlickova L;Li Y;Trick M;Fraser F;Wang L;Fellgett A;Sollars ES;Janacek SH;Downie JA;Buggs RJ;Kjær ED;Bancroft I
树木疾病流行是一个全球性问题，影响到粮食安全、生物多样性和国家经济。树木的保护和繁殖潜力受到复杂基因组和长生命周期的阻碍，大多数物种缺乏基因组资源。欧洲白蜡树被真菌病原体Hymenoscyphus fraxineus摧毁，这种病原体会导致白蜡树枯死。以该系统为例，并首次在植物病理学研究中利用关联转录组学，我们发现了基因序列和基因表达变体，这些基因序列和基因表达变体跨越了遗传多样性面板，这些面板针对疾病症状进行评分，并确定了与受感染树木中的冠层损伤密切相关的标记。利用这些标记，我们预测了一组无关树木的表型，成功地识别出了对疾病易感性低的个体。共表达分析表明，预启动的防御反应可能是降低对白蜡枯梢病的敏感性的基础。
Tree disease epidemics are a global problem, impacting food security, biodiversity and national economies. The potential for conservation and breeding in trees is hampered by complex genomes and long lifecycles, with most species lacking genomic resources. The European Ash tree Fraxinus excelsior is being devastated by the fungal pathogen Hymenoscyphus fraxineus, which causes ash dieback disease. Taking this system as an example and utilizing Associative Transcriptomics for the first time in a plant pathology study, we discovered gene sequence and gene expression variants across a genetic diversity panel scored for disease symptoms and identified markers strongly associated with canopy damage in infected trees. Using these markers we predicted phenotypes in a test panel of unrelated trees, successfully identifying individuals with a low level of susceptibility to the disease. Co-expression analysis suggested that pre-priming of defence responses may underlie reduced susceptibility to ash dieback.