THE BASOLATERAL DOMAIN OF THE HEPATOCYTE PLASMA-MEMBRANE BEARS RECEPTORS FOR THE CIRCUMSPOROZOITE PROTEIN OF PLASMODIUM-FALCIPARUM SPOROZOITES
THE BASOLATERAL DOMAIN OF THE HEPATOCYTE PLASMA-MEMBRANE BEARS RECEPTORS FOR THE CIRCUMSPOROZOITE PROTEIN OF PLASMODIUM-FALCIPARUM SPOROZOITES
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DOI：
10.1016/0092-8674(92)90251-7
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发表时间：
1992-09-18
期刊：
影响因子：
64.5
通讯作者：
NUSSENZWEIG, V
中科院分区：
文献类型：
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作者：
CERAMI, C;FREVERT, U;NUSSENZWEIG, V
疟疾子孢子注入循环后几分钟进入肝细胞。入侵过程的速度和特异性表明它是受体介导的。我们在这里表明，重组恶性疟原虫环子孢子蛋白（CS）特异性结合到区域的肝细胞的质膜暴露于循环血液中的Disse空间。在其他器官中，甚至在肝细胞膜的其他区域中均未检测到结合。CS与肝细胞的相互作用，以及子孢子侵入HepG2细胞，被抑制的合成肽代表进化保守区II的CS。我们得出结论，区域II是肝细胞受体的子孢子配体定位于质膜的基底外侧域。我们的研究结果为疟疾子孢子的靶细胞特异性提供了合理的解释。
Minutes after injection into the circulation, malaria sporozoites enter hepatocytes. The speed and specificity of the invasion process suggest that it is receptor mediated. We show here that recombinant Plasmodium falciparum circumsporozoite protein (CS) binds specifically to regions of the plasma membrane of hepatocytes exposed to circulating blood in the Disse space. No binding has been detected in other organs, or even in other regions of the hepatocyte membrane. The interaction of CS with hepatocytes, as well as sporozoite invasion of HepG2 cells, is inhibited by synthetic peptides representing the evolutionarily conserved region II of CS. We conclude that region II is a sporozoite ligand for hepatocyte receptors localized to the basolateral domain of the plasma membrane. Our findings provide a rational explanation for the target cell specificity of malaria sporozoites.